Invitrogen

Protocadherin Gamma Monoclonal Antibody (N159/5)

Name: Protocadherin Gamma Monoclonal Antibody (N159\/5)
Brand: Invitrogen
SKU: MA5-27615

查看其他15个Protocadherin Gamma抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (2)

Protocadherin Gamma Antibody in Immunocytochemistry (ICC/IF)

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Protocadherin Gamma Antibody (MA5-27615) in ICC/IF

Immunofluorescent analysis of Protocadherin Gamma in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with Protocadherin Gamma monoclonal antibody (Product # MA5-27615) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:200, 1:1000 (60 min at RT) and 1:5000 ... View More

Protocadherin Gamma Antibody in Western Blot (WB)

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产品信息

MA5-27615

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组织化学（多聚甲醛固定） (IHC (PFA))

1:100

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

N159/5

抗原

Fusion protein corresponding to amino acids 808-931 (C-terminal cytoplasmic constant domain) of mouse Protocadherin-gamma-A1 that is shared by all 22 Gamma-protocadherins (A subfamily amino acids ~807-930, B subfamily amino acids ~789-912 and C subfamily amino acids ~818-941). 99% identity with human (123/124 amino acids).

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2735332

产品详细信息

1 µg/mL of MA5-27615 was sufficient for detection of Protocadherin gamma (pan) in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 100kDa. Cross-reacts with all Gamma-protocadherin-A, -B and -C proteins.

This antibody was formerly sold as clone S159-5.

靶标信息

The protocadherin gamma gene cluster is one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 3' RACE product; cadherin 20; cadherin 21; cadherin ME3; cadherin ME6; Cadherin-20; Cadherin-21; cadherin-like 2; fibroblast cadherin FIB2; fibroblast cadherin FIB3; Fibroblast cadherin-2; Fibroblast cadherin-3; Pan-Gamma-Protocadherin-A; PC-43; PCDH gamma A; PCDH-gamma-A; PCDH-gamma-A1; PCDH-gamma-A10; PCDH-gamma-A11; PCDH-gamma-A12; PCDH-gamma-A2; PCDH-gamma-A3; PCDH-gamma-A4; PCDH-gamma-A5; PCDH-gamma-A6; PCDH-gamma-A7; PCDH-gamma-A8; PCDH-gamma-A9; PCDH-gamma-B1; PCDH-gamma-B2; PCDH-gamma-B3; PCDH-gamma-B4; PCDH-gamma-B5; PCDH-gamma-B6; PCDH-gamma-B7; PCDH-gamma-C3; PCDH-gamma-C4; PCDH-gamma-C5; PCDHGA; pcdhga4; protocadherin 13; protocadherin 2; protocadherin 43; protocadherin gamma a1; protocadherin gamma A12; protocadherin gamma a2; protocadherin gamma B2; protocadherin gamma B6; protocadherin gamma b7; protocadherin gamma C-VI; protocadherin gamma c5; protocadherin gamma subfamily A, 10; protocadherin gamma subfamily A, 12; protocadherin gamma subfamily A, 3; protocadherin gamma subfamily A, 4; protocadherin gamma subfamily B, 1, isoform 2; protocadherin gamma subfamily B, 5; protocadherin gamma subfamily B, 7; protocadherin gamma subfamily C, 3; Protocadherin gamma-A1; Protocadherin gamma-A10; Protocadherin gamma-A11; Protocadherin gamma-A12; Protocadherin gamma-A2; Protocadherin gamma-A3; Protocadherin gamma-A4; Protocadherin gamma-A5; Protocadherin gamma-A6; protocadherin gamma-A6-like; Protocadherin gamma-A7; Protocadherin gamma-A8; Protocadherin gamma-A9; Protocadherin gamma-B1; Protocadherin gamma-B2; Protocadherin gamma-B3; Protocadherin gamma-B4; protocadherin gamma-B4-like; Protocadherin gamma-B5; Protocadherin gamma-B6; protocadherin gamma-B6-like; Protocadherin gamma-B7; Protocadherin gamma-C3; Protocadherin gamma-C4; Protocadherin gamma-C5; Protocadherin-2; Protocadherin-43

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基因别名: CDH-GAMMA-A5; CDH20; CDH21; FIB2; FIB3; KIAA0327; KIAA0588; ME3; ME6; mKIAA0588; mKIAA4054; pc2c; PC43; PCDH-GAMMA-A1; PCDH-GAMMA-A10; PCDH-GAMMA-A11; PCDH-GAMMA-A12; PCDH-GAMMA-A2; PCDH-GAMMA-A3; PCDH-GAMMA-A4; PCDH-GAMMA-A5; PCDH-GAMMA-A6; PCDH-GAMMA-A7; PCDH-GAMMA-A8; PCDH-GAMMA-A9; PCDH-GAMMA-B1; PCDH-GAMMA-B2; PCDH-GAMMA-B3; PCDH-GAMMA-B4; PCDH-GAMMA-B5; PCDH-GAMMA-B6; PCDH-GAMMA-B7; PCDH-GAMMA-C3; PCDH-GAMMA-C4; PCDH-GAMMA-C5; Pcdh13; PCDH2; PCDHGA1; PCDHGA10; Pcdhga10temp; PCDHGA11; PCDHGA12; PCDHGA2; PCDHGA3; PCDHGA4; PCDHGA5; PCDHGA6; PCDHGA7; PCDHGA8; PCDHGA9; PCDHGB1; PCDHGB2; PCDHGB3; PCDHGB4; PCDHGB5; PCDHGB6; PCDHGB7; PCDHGC3; PCDHGC4; PCDHGC5; UNQ371/PRO707