Invitrogen

RAB6A Polyclonal Antibody

查看其他9个RAB6A抗体

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RAB6A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of RAB6A in paraffin-embedded section of rat small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-RAB6A antibody (Product # PA5-95528) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

RAB6A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RAB6A in paraffin-embedded human placenta tissues. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with RAB6A polyclonal antibody (Product # PA5-95528) at a 2 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

RAB6A Antibody in Western Blot (WB) — Western blot analysis of RAB6A in Lane 1: human MDA-MB-453 whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse HEPA1-6 whole cell lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with RAB6A polyclonal antibody (Product # PA5-95528) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).

产品信息

PA5-95528

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

-

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence of human RAB6A (RRVAAALPGMESTQDRSREDMIDIKLEKPQEQPVSE).

查看免疫原

偶联物

Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2807330

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

Rab proteins are also an integral part of endocytic pathways. Rab 6A is a ubiquitously expressed member of the Rab family of proteins and localizes to the Golgi membrane where it regulates retrograde transport from the late endosomes via the Golgi to endoplasmic reticulum (ER) pathway. Three isoforms exist due to alternative splicing events, namely the ubiquitously expressed isoforms Rab 6A' and Rab 6A, and the brain-specific isoform Rab 6B.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Rab GTPase; Rab-6; RAB6, member RAS oncogene family; RAB6B, member RAS oncogene family; Ras-related protein Rab-6A

基因别名: 2610028L11Rik; AA419671; MNCb-1660; RAB6; RAB6A; Rab6b; RCO4-3

UniProt ID: (Human) P20340, (Rat) Q9WVB1, (Mouse) P35279

Entrez Gene ID: (Human) 5870, (Rat) 84379, (Mouse) 19346

protein targeting to Golgi retrograde vesicle-mediated transport, Golgi to ER small GTPase mediated signal transduction viral process peptidyl-cysteine methylation antigen processing and presentation protein localization to Golgi apparatus early endosome to Golgi transport minus-end-directed organelle transport along microtubule intracellular protein transport intra-Golgi vesicle-mediated transport metabolic process Rab protein signal transduction positive regulation of ATPase activity retrograde transport, endosome to Golgi Golgi vesicle transport transport protein transport vesicle-mediated transport

参与通路

GTPase activity protein binding GTP binding protein domain specific binding myosin V binding ATPase activator activity GDP binding ATPase binding nucleotide binding