Invitrogen

RAB7A Recombinant Rabbit Monoclonal Antibody (SN202-03)

RAB7A Antibody in Immunocytochemistry (ICC/IF)

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RAB7A Antibody (MA5-32340) in ICC/IF

Immunofluorescence of RAB7A was performed using HeLa WT and RAB7A KO cells that were labeled with a green or a far-red fluorescent dye, respectively. Post-labeling, WT and KO cells were mixed and plated to a 1:1 ratio in a 96-well plate with an optically clear flat bottom as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) for 15 min at RT; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS containing 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min at RT. Cells were stained ... View More

RAB7A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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RAB7A Antibody — Antibody specificity was demonstrated by knockout (KO) of target protein. Loss of signal in immunofluorescence was observed for target protein in RAB7A KO cell line using RAB7A Recombinant Rabbit Monoclonal Antibody (SN202-03) (Product # MA5-32340). Parental cells were labelled with CellTracker™ Green CMFDA Dye (Product # C2925) and DDX1 KO cells with CellTracker™ Deep Red Dye (Product # C34565). Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockdown/knockdown validation. {KO}

产品信息

MA5-32340

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000

免疫组化（石蜡） (IHC (P))

1:50-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1000

流式细胞分析 (Flow)

1:500-1:1,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

SN202-03

抗原

Synthetic peptide within Human RAB7 aa 158-207

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2809621

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: rab-7; RAB7, member RAS oncogene family; Ras-associated protein RAB7; Ras-related protein BRL-RAS; Ras-related protein p23; Ras-related protein Rab-7a

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基因别名: PRO2706; RAB7; RAB7A

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UniProt ID: (Human) P51149, (Mouse) P51150, (Rat) P09527

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Entrez Gene ID: (Human) 7879, (Mouse) 19349, (Rat) 29448

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功能

GTPase activity protein binding GTP binding GDP binding Rac GTPase binding nucleotide binding

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