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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • RAD51 Antibodies

          Invitrogen

          RAD51 Monoclonal Antibody (14B4)

          高级验证
          3个已发表图片
          3 篇参考文献
          查看其他33个RAD51抗体

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          说明书
          实验方案
          问题与答案
          技术支持
          说明书
          实验方案
          问题与答案
          技术支持

          Cite RAD51 Monoclonal Antibody (14B4)

          • 抗体检测数据 (10)
          • 已发表图片 (3)
          • 高级验证 (2)
          RAD51 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          RAD51 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 15

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          RAD51 Antibody (MA1-23271) in ICC/IF

          Immunofluorecent staning of RAD51 nuclear foci in U2OS cells using RAD51 14B4 antibody (Product # MA1-23271). Cells were pre-extracted with CSK buffer before fixation with 4% PFA. RAD51 14B4 was used at 1:1,000 dultion. DAPI was used to counterstain the nucleus. Scale bar, 10 mciro-meter. {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

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          RAD51 Antibody in Immunocytochemistry (ICC/IF)
          RAD51 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          RAD51 Antibody in Western Blot (WB)
          RAD51 Antibody in Western Blot (WB)
          RAD51 Antibody in Western Blot (WB)
          RAD51 Antibody in Western Blot (WB)
          RAD51 Antibody in Western Blot (WB)
          RAD51 Antibody in Western Blot (WB)
          RAD51 Antibody in Western Blot (WB)
          RAD51 Antibody in Immunoprecipitation (IP)
          RAD51 Antibody in Immunocytochemistry (ICC/IF)
          RAD51 Antibody in Immunocytochemistry (ICC/IF)
          RAD51 Antibody in Flow Cytometry (Flow)
          RAD51 Antibody
          RAD51 Antibody

          产品信息

          MA1-23271

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:500-1:3,000
          -

          免疫组化(石蜡) (IHC (P))

          1:100-1:1,000
          -

          免疫细胞化学 (ICC/IF)

          1:100-1:1,000
          查看2篇已发表文章 2篇已发表文章

          流式细胞分析 (Flow)

          -
          查看1篇已发表文章 1篇已发表文章

          免疫沉淀 (IP)

          Assay-dependent
          -

          in situ PLA (PLA)

          Assay-dependent
          -
          产品规格

          种属反应

          Chicken, Human, Mouse, Rat

          已发表种属

          Human

          宿主/亚型

          Mouse / IgG2a

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          14B4

          抗原

          Full length (amino acids 1-338) Rad51 expressed in E. coli.
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          1.0 mg/mL

          纯化类型

          Antigen affinity chromatography

          保存液

          PBS, pH 7

          内含物

          no preservative

          保存条件

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_560832

          产品详细信息

          A suggested positive control for this product is T24.

          靶标信息

          RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: BRCA1/BRCA2-containing complex, subunit 5; DNA repair protein RAD51 homolog 1; homolog to S.cerevisiae; HsRAD51; RAD51 homolog; RAD51 homolog (RecA homolog, E. coli); RAD51 homolog A; RecA, E. coli, homolog of; RecA-like protein; recombination protein A

          查看更多 收起

          基因别名: AV304093; BRCC5; FANCR; HRAD51; HsRad51; HsT16930; MRMV2; RAD51; RAD51A; RECA; RGD1563603

          查看更多 收起

          UniProt ID: (Human) Q06609, (Chicken) P37383, (Mouse) Q08297

          查看更多 收起

          Entrez Gene ID: (Human) 5888, (Chicken) 396086, (Mouse) 19361, (Rat) 499870

          查看更多 收起

          功能
          recombinase activity four-way junction DNA binding chromatin binding double-stranded DNA binding single-stranded DNA binding endodeoxyribonuclease activity protein binding ATP binding protein C-terminus binding identical protein binding single-stranded DNA-dependent ATPase activity DNA polymerase binding DNA-dependent ATPase activity single-stranded DNA-dependent ATP-dependent DNA helicase activity nucleotide binding DNA binding damaged DNA binding molecular_function DNA metabolism protein
          参与通路
          telomere maintenance via recombination double-strand break repair via homologous recombination DNA recombinase assembly DNA synthesis involved in DNA repair strand displacement regulation of protein phosphorylation DNA unwinding involved in DNA replication DNA repair DNA recombination mitotic recombination cellular response to DNA damage stimulus meiotic nuclear division reciprocal meiotic recombination response to toxic substance response to X-ray regulation of double-strand break repair via homologous recombination telomere maintenance via telomere lengthening replication fork processing strand invasion response to drug positive regulation of DNA ligation protein homooligomerization chromosome organization involved in meiotic cell cycle cellular response to ionizing radiation cellular response to gamma radiation cellular response to hydroxyurea cellular response to cisplatin cellular response to camptothecin response to etoposide replication-born double-strand break repair via sister chromatid exchange mitotic recombination-dependent replication fork processing response to ionizing radiation interstrand cross-link repair meiotic cell cycle DNA metabolic process telomere organization cellular response to alkaloid
          It has to be done as per old AB suggested Products section.
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          只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符,您即可享受免费退换货服务。*

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