Invitrogen

RAD51 Monoclonal Antibody (14B4)

RAD51 Antibody in Immunocytochemistry (ICC/IF)

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RAD51 Antibody (MA1-23271) in ICC/IF

Immunofluorecent staning of RAD51 nuclear foci in U2OS cells using RAD51 14B4 antibody (Product # MA1-23271). Cells were pre-extracted with CSK buffer before fixation with 4% PFA. RAD51 14B4 was used at 1:1,000 dultion. DAPI was used to counterstain the nucleus. Scale bar, 10 mciro-meter.

RAD51 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAD51 Antibody in Immunoprecipitation (IP)

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Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. 293T cells were transfected with RAD51 siRNA and decrease in signal intensity was observed in western blot application using RAD51 Antibody (Product # MA1-23271). {KD}

产品信息

MA1-23271

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

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流式细胞分析 (Flow)

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免疫沉淀 (IP)

Assay-dependent

in situ PLA (PLA)

Assay-dependent

产品规格

种属反应

Chicken, Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

14B4

抗原

Full length (amino acids 1-338) Rad51 expressed in E. coli.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_560832

产品详细信息

A suggested positive control for this product is T24.

靶标信息

RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

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Scientific reports

Song PN,Lynch SE,DeMellier CT,Mansur A,Gallegos CA,Wright BD,Hartman YE,Minton LE,Lapi SE,Warram JM,Sorace AG

Published figure using RAD51 monoclonal antibody (Product # MA1-23271) in Flow Cytometry

Wed Feb 14 00:00:00 UTC 2024

Alterations in the p53 isoform ratio govern breast cancer cell fate in response to DNA damage.

Cell death & disease

Steffens Reinhardt L,Zhang X,Groen K,Morten BC,De Iuliis GN,Braithwaite AW,Bourdon JC,Avery-Kiejda KA

MA1-23271 was used in Immunocytochemistry-immunofluorescence to suggest that the response of breast cancer cells to standard of care DNA-damaging therapies is dependent on the expression of p53 isoforms, which may contribute to outcomes in breast cancer.

Fri Oct 28 00:00:00 UTC 2022

A localized nucleolar DNA damage response facilitates recruitment of the homology-directed repair machinery independent of cell cycle stage.

Genes & development

van Sluis M,McStay B

MA1-23271 was used in immunocytochemistry to study the response to double-stranded breaks at the nucleolar organizer regions.

Mon Jun 01 00:00:00 UTC 2015

生物信息学

蛋白别名: BRCA1/BRCA2-containing complex, subunit 5; DNA repair protein RAD51 homolog 1; homolog to S.cerevisiae; HsRAD51; RAD51 homolog; RAD51 homolog (RecA homolog, E. coli); RAD51 homolog A; RecA, E. coli, homolog of; RecA-like protein; recombination protein A

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基因别名: AV304093; BRCC5; FANCR; HRAD51; HsRad51; HsT16930; MRMV2; RAD51; RAD51A; RECA; RGD1563603

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UniProt ID: (Human) Q06609, (Chicken) P37383, (Mouse) Q08297

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Entrez Gene ID: (Human) 5888, (Chicken) 396086, (Mouse) 19361, (Rat) 499870

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功能