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Invitrogen
RAR gamma-1 Polyclonal Antibody
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RAR gamma-1 Antibody (PA3-813) in WB
Western blot was performed using Anti-RAR gamma-1 Polyclonal Antibody (Product # PA3-813) and a 60 kDa band corresponding to RAR gamma-1 was observed in MCF-7, HaCaT and Mouse Mammary Gland but not in BeWo and HepG2. Nuclear enriched extracts (50 µg lysate) of MCF7 (Lane 1), BeWo (Lane 2), HaCaT (Lane 3), Hep G2 (Lane 4) and tissue extract (50 µg lysate) of Mouse Mammary gland (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (P... View More
产品信息
PA3-813
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to the residues E(40) M L S P S F R G L G Q P D L P K E(57) of the N-terminal end of RAR gamma-1.
偶联物
形式
Liquid
浓度
Conc. Not Determined
保存液
whole serum, PBS
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
PA3-813 detects recombinant human retinoic acid receptor (RAR) gamma-1. The immunizing sequence is 100% conserved in mouse. This antibody detects RAR gamma-1, but not RAR gamma-2, or either of the alpha or beta forms of RAR.
PA3-813 has been successfully used in Western blot, immunoprecipitation and gel shift procedures. By Western blot, this antibody detects an ~50 kDa protein representing RAR gamma-1 from transiently transfected 293T cells. PA3-813 has been shown to efficiently super shift the human RAR gamma-1/retinoid X receptor (RXR)/RAR response element complex in gel super shift experiments using a number of different response elements.
PA3-813 immunizing peptide corresponds to the N-terminus of RAR gamma-1. This peptide (Cat. # PEP-228) is available for use in neutralization and control experiments.
靶标信息
Retinoic acid, a metabolite of vitamin A, is necessary for normal organogenesis but acts as a teratogen at high levels during embryonic and fetal development. Retinoic acid functions through its interaction with the nuclear protein, retinoic acid receptor (RAR). RAR belongs to the steroid and thyroid hormone superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. RAR exists as three major subtypes: alpha, beta and gamma. Two main isoforms of RAR gamma exist: RAR gamma-1 has been found to be predominantly expressed in skin, while RAR gamma-2 has been found early in embryogenesis and in embryonal carcinoma cells. RAR gamma is also known as RARD. The corresponding gene for the retinoic acid receptor gamma 1 is NR1B3.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Nuclear receptor subfamily 1 group B member 3; RAR gamma 2; RAR Gamma-1; RAR-gamma; retinoic acid; retinoic acid nuclear receptor gamma variant 1; retinoic acid nuclear receptor gamma variant 2; retinoic acid receptor; Retinoic acid receptor gamma; retinoic acid receptor gamma 4; retinoic acid receptor, gamma
基因别名: NR1B3; RAR-gamma; RARC; RARD; RARG; RARgamma2
UniProt ID: (Human) P13631, (Mouse) P18911
Entrez Gene ID: (Human) 5916, (Mouse) 19411, (Rat) 685072
RNA polymerase II regulatory region sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
retinoic acid receptor activity
protein binding
zinc ion binding
retinoid X receptor binding
sequence-specific DNA binding
metal ion binding
C4 zinc finger nuclear receptor
negative regulation of transcription from RNA polymerase II promoter
neural tube closure
glandular epithelial cell development
retinal pigment epithelium development
growth plate cartilage chondrocyte growth
transcription initiation from RNA polymerase II promoter
positive regulation of cell proliferation
negative regulation of cell proliferation
regulation of cell size
anterior/posterior pattern specification
embryonic camera-type eye development
regulation of myelination
negative regulation of chondrocyte differentiation
response to retinoic acid
embryonic hindlimb morphogenesis
multicellular organism growth
positive regulation of apoptotic process
negative regulation of apoptotic process
positive regulation of programmed cell death
steroid hormone mediated signaling pathway
regulation of myeloid cell differentiation
positive regulation of transcription from RNA polymerase II promoter
embryonic eye morphogenesis
retinoic acid receptor signaling pathway
canonical Wnt signaling pathway
face development
trachea cartilage development
prostate gland epithelium morphogenesis
Harderian gland development
cellular response to retinoic acid
chondrocyte development
growth plate cartilage development
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
regulation of gene expression
positive regulation of gene expression
camera-type eye development
negative regulation of cell differentiation
reproductive structure development
gland development
retina development in camera-type eye
limb development
bone development
bone morphogenesis
epithelium development
negative regulation of cartilage development
