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Invitrogen
RAR gamma-2 Polyclonal Antibody
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RAR gamma-2 Antibody (PA3-814) in WB
Western blot analysis of Retinoic Acid Receptor gamma-2 was performed by loading 25 µg of SK-BR-3 (lane 1) and Hela (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a Retinoic Acid Receptor gamma-2 polyclonal antibody (Product # PA3-814) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed usin... View More
产品信息
PA3-814
应用
建议稀释比
已发表文章
产品规格
已发表种属
Human,
Non-human primate
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues R(13) R L Y G A A G P G A G L L R R(28) of the N-terminal sequence of RAR gamma-2.
偶联物
形式
Liquid
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
产品详细信息
PA3-814 detects recombinant human retinoic acid receptor (RAR) gamma 2. The immunizing sequence is 100% conserved in mouse. This antibody does not detect RAR gamma-1, or either of the alpha or beta forms of RAR.
PA3-814 has been successfully used in WB, immunoprecipitation and gel shift procedures. In super shift assays this antibody incompletely shifts human RAR gamma-2/retinoid X receptor (RXR)/RAR response element complexes using various synthetic and natural RARE's.
PA3-814 immunizing peptide corresponds to the N-terminus of RAR gamma-2. This antibody does not detect RAR gamma-1, or either of the alpha or beta forms of RAR. This peptide (Cat. # PEP-229) is available for use in neutralization and control experiments.
靶标信息
Retinoic acid, a metabolite of vitamin A, is necessary for normal organogenesis but acts as a teratogen at high levels during embryonic and fetal development. Retinoic acid functions through its interaction with the nuclear protein, retinoic acid receptor (RAR). RAR belongs to the steroid and thyroid hormone superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. RAR exists as three major subtypes: alpha, beta and gamma. Two main isoforms of RAR gamma exist: RAR gamma-1 has been found to be predominantly expressed in skin, while RAR gamma-2 has been found early in embryogenesis and in embryonal carcinoma cells. RAR gamma is also known as RARD.||The corresponding gene for the retinoic acid receptor gamma 2 is NR1B3.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
