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Invitrogen
RFC3 Polyclonal Antibody
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RFC3 Antibody (PA1-27673)
RFC3 knockdown impairs the G1/S cell cycle progression. KG1 cells were transduced with pLKO.1 lentiviral vectors expressing RFC3 shRNA or luciferase shRNA, and then transduced cells were selected with puromycin. (a) Suppressed expression of RFC3 by corresponding specific shRNA was assessed at protein level. Total cell lysates were analyzed by immunoblotting for RFC3. ?-tubulin was used as a loading control.... View More
产品信息
PA1-27673
产品规格
已发表种属
Human
宿主/亚型
Chicken
/ IgY
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to human Replication factor C subunit 3.
偶联物
形式
Liquid
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
PA1-27673 detects RFC3 from human and rat samples.
PA1-27673 may be successfully used in ELISA and Western blot applications. By Western blot, this antibody has only been tested on fusion protein.
The immunogen is a synthetic peptide corresponding to human Replication factor C subunit 3.
靶标信息
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 38 kD subunit. This subunit is essential for the interaction between the 140 kD subunit and the core complex that consists of the 36, 37, and 40 kD subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
