Invitrogen

RFC4 Polyclonal Antibody

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RFC4 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of RFC4 was performed in U2OS cells using RFC4 Polyclonal Antibody (Product # PA5-96340).

RFC4 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of RFC4 in paraffin-embedded human breast cancer using RFC4 Polyclonal Antibody (Product # PA5-96340) at a dilution of 1:100.

RFC4 Antibody in Western Blot (WB) — Western Blot analysis of RFC4 in extracts of various cell lines using RFC4 Polyclonal Antibody (Product # PA5-96340) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

RFC4 Antibody in Immunoprecipitation (IP) — Immunoprecipitation analysis of RFC4 was performed in 200 µg extracts of K562 cells using RFC4 Polyclonal Antibody (Product # PA5-96340). Western blot was performed from the immunoprecipitate using RFC4 Polyclonal Antibody.

产品信息

PA5-96340

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:2,000	-
免疫组化（石蜡） (IHC (P))	1:50 - 1:200	-
免疫细胞化学 (ICC/IF)	1:50 - 1:100	-
免疫沉淀 (IP)	1:50 - 1:200	-

产品规格
种属反应	Human, Rat
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Recombinant fusion protein containing a sequence corresponding to amino acids 204-363 of human RFC4.
偶联物	Unconjugated
形式	Liquid
浓度	0.69 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.3, with 50% glycerol
内含物	0.02% sodium azide
保存条件	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2808142

产品详细信息

Immunogen sequence: DKIQQQRLLD IAKKENVKIS DEGIAYLVKV SEGDLRKAIT FLQSATRLTG GKEITEKVIT DIAGVIPAEK IDGVFAACQS GSFDKLEAVV KDLIDEGHAA TQLVNQLHDV VVENNLSDKQ KSIITEKLAE VDKCLADGAD EHLQLISLCA TVMQQLSQNC; Positive Samples: HeLa, SW480, 22Rv1, SW620, K-562, HepG2, MCF7; Cellular Location: Nucleus

靶标信息

Human Replication Factor C (RFC) is a hetero-pentameric protein consisting of the p140, p40, p38, p37 and p36 subunits. RFC functions in the loading of the DNA polymerase delta processivity factor (PCNA, Proliferating Cell nuclear Antigen) onto a primed DNA template during DNA synthesis and repair. RFC may also participate in the DNA damage checkpoint response with a PCNA-like hetero-trimer consisting of Hus 1, Rad 1 and Rad 9.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: A1 37 kDa subunit; Activator 1 37 kDa subunit; Activator 1 subunit 4; replication factor C (activator 1) 4, 37kDa; Replication factor C 37 kDa subunit; Replication factor C subunit 4; RF-C 37 kDa subunit; RFC 37 kDa subunit; RFC p37

基因别名: A1; RFC37; RFC4

UniProt ID: (Human) P35249

Entrez Gene ID: (Human) 5984, (Rat) 288003

分子生物学功能: DNA-directed DNA polymerase