应用 | 建议稀释比 | 已发表文章 |
---|---|---|
免疫印迹 (WB) |
1:500-1:2,000 | - |
免疫组化(石蜡) (IHC (P)) |
1:50 - 1:200 | - |
免疫细胞化学 (ICC/IF) |
1:50 - 1:100 | - |
免疫沉淀 (IP) |
1:50 - 1:200 | - |
产品规格 | |
---|---|
种属反应 |
Human, Rat |
宿主/亚型 |
Rabbit / IgG |
分类 |
Polyclonal |
类型 |
Antibody |
抗原 |
Recombinant fusion protein containing a sequence corresponding to amino acids 204-363 of human RFC4. |
偶联物 |
Unconjugated |
形式 |
Liquid |
浓度 |
0.69 mg/mL |
纯化类型 |
Antigen affinity chromatography |
保存液 |
PBS, pH 7.3, with 50% glycerol |
内含物 |
0.02% sodium azide |
保存条件 |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2808142 |
Immunogen sequence: DKIQQQRLLD IAKKENVKIS DEGIAYLVKV SEGDLRKAIT FLQSATRLTG GKEITEKVIT DIAGVIPAEK IDGVFAACQS GSFDKLEAVV KDLIDEGHAA TQLVNQLHDV VVENNLSDKQ KSIITEKLAE VDKCLADGAD EHLQLISLCA TVMQQLSQNC; Positive Samples: HeLa, SW480, 22Rv1, SW620, K-562, HepG2, MCF7; Cellular Location: Nucleus
Human Replication Factor C (RFC) is a hetero-pentameric protein consisting of the p140, p40, p38, p37 and p36 subunits. RFC functions in the loading of the DNA polymerase delta processivity factor (PCNA, Proliferating Cell nuclear Antigen) onto a primed DNA template during DNA synthesis and repair. RFC may also participate in the DNA damage checkpoint response with a PCNA-like hetero-trimer consisting of Hus 1, Rad 1 and Rad 9.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: A1 37 kDa subunit; Activator 1 37 kDa subunit; Activator 1 subunit 4; replication factor C (activator 1) 4, 37kDa; Replication factor C 37 kDa subunit; Replication factor C subunit 4; RF-C 37 kDa subunit; RFC 37 kDa subunit; RFC p37
基因别名: A1; RFC37; RFC4
UniProt ID: (Human) P35249
Entrez Gene ID: (Human) 5984, (Rat) 288003
分子生物学功能:
DNA-directed DNA polymerase