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产品信息
PA5-50121
| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
1:500-1:3,000 | - |
| 产品规格 | |
|---|---|
种属反应 |
Human |
宿主/亚型 |
Rabbit / IgG |
分类 |
Polyclonal |
类型 |
Antibody |
抗原 |
Synthesized peptide derived from internal of human RFWD3. |
偶联物 |
Unconjugated |
形式 |
Liquid |
浓度 |
1 mg/mL |
纯化类型 |
Antigen affinity chromatography |
保存液 |
PBS, pH 7.4, with 50% glycerol |
内含物 |
0.02% sodium azide |
保存条件 |
-20°C |
RRID |
AB_2635574 |
产品详细信息
The antibody detects endogenous levels of total RFWD3 protein.
靶标信息
E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658). Plays a key role in RPA-mediated DNA damage signaling and repair (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658, PubMed:28691929). Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination (PubMed:26474068, PubMed:28575657, PubMed:28575658). Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint (PubMed:20173098). May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome (PubMed:20173098). In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53 (PubMed:20173098). [UniProt]
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: E3 ubiquitin-protein ligase RFWD3; RING finger and WD repeat domain-containing protein 3; RING finger protein 201
基因别名: RFWD3; RNF201
UniProt ID: (Human) Q6PCD5
Entrez Gene ID: (Human) 55159
分子生物学功能:
ubiquitin-protein ligase
