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Invitrogen
RIP140 Polyclonal Antibody
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RIP140 Antibody (PA1-841) in ICC/IF
Immunofluorescence analysis of RIP140 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with RIP140 Polyclonal Antibody (Product # PA1-841) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731... View More
产品信息
PA1-841
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Non-human primate,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues G(718) N P T K G R V K K K E K T P L R D(735) of human RIP140.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
PA1-841 detects recombinant human Receptor-Interacting Protein 140 (RIP 140).
PA1-841 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects two proteins at ~140 kDa representing human RIP 140 expressed in rabbit reticulocyte lysate.
The PA1-841 immunogen is a synthetic peptide corresponding to residues G(718) N P T K G R V K K K E K T P L R D(735) of human RIP140. This immunizing peptide (Cat. # PEP-030) is available for use in neutralization and control experiments.
靶标信息
Nuclear receptor interacting protein 1 is a nuclear protein that specifically interacts with the hormone-dependent activation domain AF2 of nuclear receptors. Also known as RIP140, this protein modulates transcriptional activity of the estrogen receptor.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ERAP 140; FLJ77253; Nuclear factor RIP140; Nuclear receptor-interacting protein 1; OTTHUMP00000083036; OTTHUMP00000083037; Receptor-interacting protein 140
基因别名: NRIP1; RIP140
UniProt ID: (Human) P48552
Entrez Gene ID: (Human) 8204
core promoter sequence-specific DNA binding
transcription coactivator activity
transcription corepressor activity
protein binding
estrogen receptor binding
nuclear hormone receptor binding
glucocorticoid receptor binding
histone deacetylase binding
retinoid X receptor binding
androgen receptor binding
negative regulation of transcription from RNA polymerase II promoter
ovarian follicle rupture
transcription, DNA-templated
circadian rhythm
lipid storage
androgen receptor signaling pathway
circadian regulation of gene expression
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cellular response to estradiol stimulus
