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Invitrogen
RNF2 Recombinant Rabbit Monoclonal Antibody (13H1L14)
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
图: 1 / 4
RNF2 Antibody (703252) in ICC/IF
For immunofluorescence analysis, HCT 116 cells were fixed and permeabilized for detection of endogenous RNF2 using Anti-RNF2 Recombinant Rabbit Monoclonal Antibody (Product # 703252, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of RNF2 protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Antifade Mountant with DAPI (Produc... View More
产品信息
703252
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
13H1L14
抗原
Peptides corresponding to human RNF2 [1) aa183-aa201 2) aa155-aa174]
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2762381
产品详细信息
This antibody is predicted to react with Monkey, Mouse, Rat, Pig.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
RNF2 (E3 ubiquitin-protein ligase RING2) is a ligase that mediates monoubiquitination of Lys-119 of histone H2A (H2AK119Ub). Therefore, it plays a central role in histone code and gene regulation. The ligase activity is enhanced by BMI1/PCGF4. H2AK119Ub gives a specific tag for epigeneti transcriptional repression and participates in X chromosome inactivation of female mammals. RNF2 may be involved in the initiation of both imprinted and random X inactivation. RNF2 is also an essential component of a Polycomb group (PcG) multiprotein PRC1-like complex. This complex class is required to maintain the transcriptionally repressive state of many genes including Hox genes throughout development. The PcG PRC1 complex acts via chromatin remodeling and modification of histones and rendering chromatin heritably changed in its expressibility.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: BAP 1; DinG protein; E3 ubiquitin-protein ligase RING2; GS1-120K12.1; HIP2-interacting protein 3; HIPI 3; Huntingtin-interacting protein 2-interacting protein 3; OTTHUMP00000060668; Protein DinG; RING 1B; RING 2; RING finger protein 1B; RING finger protein 2; RING finger protein BAP-1; RING-type E3 ubiquitin transferase RING2; RING1b; RNF 2
基因别名: BAP-1; BAP1; DING; HIPI3; RING1B; RING2; RNF2
UniProt ID: (Human) Q99496
Entrez Gene ID: (Human) 6045
negative regulation of transcription from RNA polymerase II promoter
mitotic cell cycle
gastrulation with mouth forming second
transcription, DNA-templated
germ cell development
anterior/posterior axis specification
protein sumoylation
spinal cord development
response to retinoic acid
histone H2A monoubiquitination
histone H2A-K119 monoubiquitination
negative regulation of sequence-specific DNA binding transcription factor activity
