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ROBO4 Antibody (PA5-97279) in WB
Western blot analysis of ROBO4 in extracts of various cell lines. Samples were incubated with ROBO4 Polyclonal antibody (Product # PA5-97279) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
产品信息
PA5-97279
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 848-1007 of human ROBO4 (NP_0619284)
偶联物
形式
Liquid
浓度
0.85 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2809081
产品详细信息
Immunogen sequence: GVGPKGGVLL CPPRPCLTPT PSEGSLANGW GSASEDNAAS ARASLVSSSD GSFLADAHFA RALAVAVDSF GFGLEPREAD CVFIDASSPP SPRDEIFLTP NLSLPLWEWR PDWLEDMEVS HTQRLGRGMP PWPPDSQISS QRSQLHCRMP KAGASPVDYS; Positive Samples: HT-29, HepG2, HT-1080, HeLa
靶标信息
Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins. Involved in the maintenance of endothelial barrier organization and function (PubMed:30455415). [UniProt]
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: FLJ20798; Magic roundabout; MGC133352; MGC133353; Roundabout homolog 4; roundabout homolog 4, magic roundabout; roundabout, axon guidance receptor, homolog 4
基因别名: ECSM4; MRB; ROBO4; UNQ421/PRO3674
UniProt ID: (Human) Q8WZ75
Entrez Gene ID: (Human) 54538
