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Invitrogen
RPA2 Recombinant Rabbit Monoclonal Antibody (JE45-59)
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RPA2 Antibody (MA5-34843) in ICC/IF
Immunofluorescence analysis of RPA2 was performed using 70% confluent log phase HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with RPA2 Recombinant Rabbit Monoclonal Antibody (JE45-59) (Product # MA5-34843) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Pl... View More
产品信息
MA5-34843
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JE45-59
抗原
Synthetic peptide within Human RPA32/RPA2 aa 221-270
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Positive Control: Mouse testis tissue, A431, SH-SY-5Y, SiHa, 293, Hela, HepG2, rat brain tissue, human colon tissue, human breast cancer tissue, human kidney tissue.
靶标信息
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
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