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Agrisera
RPB2/DNA-directed RNA polymerase II subunit 2 Polyclonal Antibody
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RPB2/DNA-directed RNA polymerase II subunit 2 Antibody (AS20-4376) in WB
10-40 µg/well of total protein extracted from 7-d-old Arabidopsis thaliana seedlings (grown in continuous light at 22ºC) with 50 m M Tris-HCl p H 7.5,150 m M Na Cl, 0.25% Triton X-100,2 m M PMSF and 1 x protease inhibitor cocktail (Roche) and denatured with 1 XLaemLi buffer at 100°C for 5 min, were separated in duplicate on 7% SDS-PAGEs and blotted 1 h 15 min to PVDF (0.2 um), using wet transfer. Blots were... View More
产品信息
AS20-4376
产品规格
种属反应
Plant
宿主/亚型
Chicken
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Arabidopsis thaliana RPB2.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: hRPB140; hs hRPB140; hsRPB2; POL2RB; rpb 2; RPB2
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