Invitrogen

RPL7 Polyclonal Antibody

查看其他8个RPL7抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (3)

RPL7 Antibody in Immunocytochemistry (ICC/IF)

RPL7 Antibody in Immunocytochemistry (ICC/IF)

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RPL7 Antibody (PA5-101391) in ICC/IF

Immunocytochemistry analysis of RPL7 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with RPL7 polyclonal antibody (Product # PA5-101391) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

RPL7 Antibody in Immunocytochemistry (ICC/IF)

RPL7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RPL7 Antibody in Western Blot (WB)

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RPL7 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of RPL7 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with RPL7 polyclonal antibody (Product # PA5-101391) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

RPL7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RPL7 in rat testis tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with RPL7 polyclonal antibody (Product # PA5-101391) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

RPL7 Antibody in Western Blot (WB) — Western blot analysis of RPL7 in extracts from various samples. Samples were incubated with RPL7 polyclonal antibody (Product # PA5-101391). Lane 1: Rat liver, blocked with antigen-specific peptides; Lane 2: Rat liver; Lane 3: Mouse brain; Lane 4: Hela cells (heat-shock treatment); Lane 5: Hepg2 cells (heat-shock treatment).

Immunogen image

RPL7 Polyclonal Antibody

Immunogen Image RPL7 Polyclonal Antibody

产品信息

PA5-101391

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human RPL7, corresponding to a region within C-terminal amino acids.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total RPL7.

靶标信息

NIP7 contains 1PUA domain and belongs to the NIP7 family. It may play a role in 60S ribosomal subunit synthesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 60S ribosomal protein L7; Large ribosomal subunit protein uL30

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基因别名: humL7-1; L7; RPL7; Rpl7a; Surf-3

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UniProt ID: (Human) P18124, (Mouse) P14148

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Entrez Gene ID: (Human) 6129, (Mouse) 19989, (Rat) 297755

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DNA binding RNA binding mRNA binding structural constituent of ribosome protein binding protein homodimerization activity poly(A) RNA binding ribosomal protein

nuclear-transcribed mRNA catabolic process, nonsense-mediated decay maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) cytoplasmic translation rRNA processing translation translational initiation SRP-dependent cotranslational protein targeting to membrane viral transcription ribosomal large subunit biogenesis