Invitrogen

RPP25L Polyclonal Antibody

查看其他4个RPP25L抗体

问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (6)

RPP25L Antibody in Immunocytochemistry (ICC/IF)

RPP25L Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

RPP25L Antibody (PA5-103588) in ICC/IF

Immunocytochemistry analysis of RPP25L in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with RPP25L polyclonal antibody (Product # PA5-103588) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

RPP25L Antibody in Immunocytochemistry (ICC/IF)

RPP25L Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RPP25L Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RPP25L Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RPP25L Antibody in Western Blot (WB)

RPP25L Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

RPP25L Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of RPP25L in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with RPP25L polyclonal antibody (Product # PA5-103588) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

RPP25L Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RPP25L in human kidney cancer and adjacent normal tissues. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with RPP25L polyclonal antibody (Product # PA5-103588) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

RPP25L Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RPP25L in mouse testis tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with RPP25L polyclonal antibody (Product # PA5-103588) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

RPP25L Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RPP25L in mouse brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with RPP25L polyclonal antibody (Product # PA5-103588) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

RPP25L Antibody in Western Blot (WB) — Western blot analysis of RPP25L in 293 cell lysate (Lane 1: treated with blocking peptide). Samples were incubated with RPP25L polyclonal antibody (Product # PA5-103588).

RPP25L Antibody in Western Blot (WB) — Western blot analysis of RPP25L in mouse brain (Lane 1: treated with blocking peptide). Samples were incubated with RPP25L polyclonal antibody (Product # PA5-103588).

Immunogen image

RPP25L Polyclonal Antibody

Immunogen Image RPP25L Polyclonal Antibody

产品信息

PA5-103588

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human RPP25L(Accession Q8N5L8), corresponding to amino acid residues C131-S163.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total C9orf23.

靶标信息

May be a component of ribonuclease P or MRP.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: alba-like protein C9orf23 homolog; Ribonuclease P protein subunit p25-like protein; RNase P protein subunit-like p25; Rpp25-like protein

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基因别名: 2810432D09Rik; AW987258; C9orf23; RPP25L; Rppl25

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UniProt ID: (Mouse) Q99JH1

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Entrez Gene ID: (Mouse) 69961

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nucleic acid binding poly(A) RNA binding endoribonuclease

biological_process