Invitrogen

RPTN Polyclonal Antibody

查看其他3个RPTN抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (3)

RPTN Antibody in Immunocytochemistry (ICC/IF)

RPTN Antibody in Immunocytochemistry (ICC/IF)

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RPTN Antibody (PA5-101837) in ICC/IF

Immunocytochemistry analysis of RPTN in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with RPTN polyclonal antibody (Product # PA5-101837) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

RPTN Antibody in Immunocytochemistry (ICC/IF)

RPTN Antibody in Immunocytochemistry (ICC/IF)

RPTN Antibody in Western Blot (WB)

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RPTN Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of RPTN in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with RPTN polyclonal antibody (Product # PA5-101837) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

RPTN Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of RPTN in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with RPTN polyclonal antibody (Product # PA5-101837) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

RPTN Antibody in Western Blot (WB) — Western blot analysis of RPTN in HeLa cell lysate (left lane: treated with the antigen-specific peptide). Samples were incubated with RPTN polyclonal antibody (Product # PA5-101837).

Immunogen image

RPTN Polyclonal Antibody

Immunogen Image RPTN Polyclonal Antibody

产品信息

PA5-101837

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human RPTN(Accession Q6XPR3), corresponding to amino acid residues V648-G698.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total RPTN.

靶标信息

Involved in the cornified cell envelope formation. Multifunctional epidermal matrix protein. Reversibly binds calcium.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: intermediate filament-associated protein; Repetin

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基因别名: RPTN

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UniProt ID: (Human) Q6XPR3

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Entrez Gene ID: (Human) 126638

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calcium ion binding