Invitrogen

RRM2 Polyclonal Antibody

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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说明书

实验方案

技术支持

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RRM2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of RRM2 in HeLa cells. Samples were incubated with RRM2 polyclonal antibody (Product # PA5-76703).

RRM2 Antibody in Western Blot (WB) — Western Blot was performed using Anti-RRM2 Polyclonal Antibody (Product # PA5-76703) and 44 kDa and 51 kDa band corresponding to Ribonucleoside-diphosphate reductase subunit M2 (RRM2) was observed across cell lines and tissues tested except in HT-29 and MCF7 which are reported low expressing for the protein. Whole cell extracts (40 µg lysate) of PANC-1 (Lane 1), HT-29 (Lane 2), MCF7 (Lane 3), MDA-MB-231 (Lane 4), HeLa (Lane 5), Mouse Thymus (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

RRM2 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of RRM2 in extracts of HeLa cells (200 µg). Samples were incubated with 1 µg of RRM2 polyclonal antibody (Product # PA5-76703).

RRM2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of RRM2 was observed in MCF7 (ER positive cell line) in comparison to MDA-MB-231 (ER negative) and HT-29 in comparison in PANC-1 using Anti-RRM2 Polyclonal Antibody (Product # PA5-76703) in Western Blot. [doi: 10.1158/1535-7163.MCT-14-0689] {RE}

产品信息

PA5-76703

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:2,000	-
免疫组化（石蜡） (IHC (P))	1:50-1:100	-
免疫细胞化学 (ICC/IF)	1:50-1:200	-
酶联免疫吸附实验 (ELISA)	1:5,000-1:10,000	-
免疫沉淀 (IP)	1:50-1:200	-

产品规格
种属反应	Human, Mouse, Rat
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Recombinant full length Human RRM2.
偶联物	Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS with 50% glycerol
内含物	0.02% sodium azide
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2720430

产品详细信息

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

靶标信息

RRM2 is one of two non-identical subunits for ribonucleotide reductase. This reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. Synthesis of the protein (M2) is regulated in a cell-cycle dependent fashion.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: ribonucleoside-diphosphate reductase M2 chain; Ribonucleoside-diphosphate reductase subunit M2; Ribonucleotide reductase 2; ribonucleotide reductase M2 polypeptide; Ribonucleotide reductase small chain; Ribonucleotide reductase small subunit; RRR2M

基因别名: AA407299; R2; RR2; RR2M; RRM2

UniProt ID: (Human) P31350, (Mouse) P11157, (Rat) Q4KLN6

Entrez Gene ID: (Human) 6241, (Mouse) 20135, (Rat) 362720

分子生物学功能: reductase