Invitrogen

RUFY1 Polyclonal Antibody

Name: RUFY1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-31400

高级验证

5个已发表图片

2 篇参考文献

查看其他12个RUFY1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

RUFY1 Antibody in Immunocytochemistry (ICC/IF)

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RUFY1 Antibody (PA5-31400) in ICC/IF

Immunocytochemistry analysis of RUFY1 in methanol-fixed MCF-7. Samples were incubated with RUFY1 polyclonal antibody (Product # PA5-31400) using a dilution of 1:500.

Product Image Gallery

RUFY1 Antibody — EGF and EGFR colocalization with EEA1 in siCON, RUFY1 KD, and PTPN23 KD cells. A-C, HeLa cells were incubated with siCON, RUFY1, or PTPN23 siRNA for 72 h prior to serum starving. The serum-starved cells were pulse-labeled with 10 ng/ml Alexa Fluor 647-EGF ligand (Invitrogen) for 0, 15, 30, 60, and 120 min, followed by fixation in 4% paraformaldehyde. The cells were permeabilized and immunostained for EEA1 and EGFR and visualized using either a goat anti-rabbit Alexa 488 or goat anti-mouse Alexa 568, respectively. Images are representative of time points from three independent experiments. The extent of colocalization between EGF or EGFR and EEA1 was measured as described under 'Experimental procedures.' The data are plotted as the percentages of colocalization for each time point. Approximately 300 cells were analyzed per time point per condition, per experiment. Scale bars, 20 µm. Images were quantified using ImageJ software. D, a representative immunoblot from each knockdown experiment, probing for PTPN23, RUFY1, and a-tubulin. For each knockdown experiment, the samples were loaded in multiple protein concentrations (20, 10, and 5 µg), and the percentage of knockdown was calculated. Only experiments with a knockdown efficiency of >90% were used. {KD}

产品信息

PA5-31400

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫细胞化学 (ICC/IF)

1:100-1:1,000

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产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human RUFY1. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2548874

产品详细信息

Recommended positive controls: IMR32, U87-MG, MCF-7, mouse adipose.

Predicted reactivity: Mouse (91%), Rat (91%), Chicken (80%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene encodes a protein that contains a RUN domain and a FYVE-type zinc finger domain. The encoded protein binds to phosphatidylinositol-3-phosphate (PI3P) and plays a role in early endosomal trafficking, tethering and fusion through interactions with small GTPases including Rab4, Rab5 and Rab14. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: FYVE-finger containing protein; FYVE-finger protein EIP1; La-binding protein 1; Rab4-interacting protein; Rab4a interacting protein; RUN and FYVE domain-containing protein 1; Zinc finger FYVE domain-containing protein 12

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基因别名: 3000002E04Rik; RABIP4; RUFY1; ZFYVE12

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UniProt ID: (Human) Q96T51, (Mouse) Q8BKQ4

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Entrez Gene ID: (Human) 80230, (Mouse) 216724

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功能

protein binding zinc ion binding lipid binding protein transporter activity metal ion binding

参与通路

endocytosis protein transport regulation of endocytosis transport