Invitrogen

RUVBL1 Polyclonal Antibody

RUVBL1 Antibody in Immunocytochemistry (ICC/IF)

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RUVBL1 Antibody (PA5-29278) in ICC/IF

Immunofluorescence analysis of RUVBL1 was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with RUVBL1 Rabbit Polyclonal Antibody (Product # PA5-29278) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) ... View More

RUVBL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RUVBL1 Antibody in Immunoprecipitation (IP)

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产品信息

PA5-29278

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

免疫沉淀 (IP)

1:100-1:500

染色质免疫沉淀实验 (ChIP)

2 µg/10^6 cells

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human RUVBL1. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.98 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2546754

产品详细信息

Recommended positive controls: A549, H1299, HCT116.

Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (97%), Xenopus laevis (97%), Chicken (99%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and four transcript variants encoding distinct isoforms have been identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

RuvBL1 Maintains Resistance to TRAIL-Induced Apoptosis by Suppressing c-Jun/AP-1 Activity in Non-Small Cell Lung Cancer.

Frontiers in oncology

Li H,Zhou T,Zhang Y,Jiang H,Zhang J,Hua Z

PA5-29278 was used in Western Blot to study the molecular mechanisms of TRAIL resistance in Non-Small Cell Lung Cancer.

Fri Jun 25 00:00:00 UTC 2021

PRMT5-Dependent Methylation of the TIP60 Coactivator RUVBL1 Is a Key Regulator of Homologous Recombination.

Molecular cell

Clarke TL,Sanchez-Bailon MP,Chiang K,Reynolds JJ,Herrero-Ruiz J,Bandeiras TM,Matias PM,Maslen SL,Skehel JM,Stewart GS,Davies CC

PA529278 was used in western blot to examine the effects of the arginine methyltransferase PRMT5 on homologous recombination-mediated double-strand break repair

Thu Mar 02 00:00:00 UTC 2017

生物信息学

蛋白别名: 49 kDa TATA box-binding protein-interacting protein; 49 kDa TBP-interacting protein; 54 kDa erythrocyte cytosolic protein; DNA helicase p50; ECP-54; INO80 complex subunit H; NMP 238; Nuclear matrix protein 238; Pontin 52; RuvB (E coli homolog)-like 1; RuvB-like 1; RuvB-like AAA ATPase 1; RuvB-like protein 1; TAP54-alpha; TATA binding protein interacting protein 49 kDa; TIP49a; TIP60-a; TIP60-associated protein 54-alpha

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基因别名: 2510009G06Rik; ECP-54; ECP54; INO80H; NMP 238; NMP238; PONTIN; Pontin52; RUVBL1; RVB1; TIH1; TIP49; TIP49A

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UniProt ID: (Human) Q9Y265, (Rat) P60123, (Mouse) P60122

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Entrez Gene ID: (Human) 8607, (Rat) 65137, (Mouse) 56505

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功能

DNA helicase activity protein binding ATP binding ATPase activity ATP-dependent 5'-3' DNA helicase activity cadherin binding involved in cell-cell adhesion ATP-dependent 3'-5' DNA helicase activity nucleotide binding helicase activity hydrolase activity

参与通路

DNA repair DNA recombination transcription, DNA-templated regulation of transcription, DNA-templated mitotic nuclear division positive regulation of plasminogen activation DNA duplex unwinding regulation of growth histone H4 acetylation histone H2A acetylation cell division regulation of fibroblast apoptotic process cellular response to DNA damage stimulus cell cycle chromatin modification regulation of mitophagy positive regulation of protein targeting to mitochondrion box C/D snoRNP assembly chromatin remodeling regulation of transcription from RNA polymerase II promoter spermatogenesis CENP-A containing nucleosome assembly cell-cell adhesion beta-catenin-TCF complex assembly positive regulation of telomerase RNA localization to Cajal body

RUVBL1 Polyclonal Antibody

RUVBL1 Antibody (PA5-29278) in ICC/IF

产品信息

PA5-29278

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

染色质免疫沉淀实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

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RUVBL1 Polyclonal Antibody

RUVBL1 Antibody (PA5-29278) in ICC/IF

产品信息

PA5-29278

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

染色质免疫沉淀 实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)