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Invitrogen
Rabbit anti-Mouse IgG2b Recombinant Secondary Antibody, PE
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Mouse IgG2b Secondary Antibody (SA5-10381-PE) in Flow
HeLa cells were fixed using IC Fixation buffer (Product # 00-8222-49). Fc block was performed using Fc Receptor Binding Inhibitor Polyclonal Antibody, eBioscience™ (Product # 14-9161-73). Cells were permeabilized using permeabilization buffer (Product # 00-8333-56) and processed using the same permeabilization buffer and stained without (blue histogram) or with TUBB4 Monoclonal Antibody (OTI5C1), TrueMAB™ (... View More
产品信息
SA5-10381-PE
应用
建议稀释比
流式细胞分析 (Flow)
0.5 µg/test
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Secondary Antibody
克隆号
26H54L63
抗原
Purified Mouse IgG
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4
内含物
5mM sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
靶标
IgG2b
抗体形式
Whole Antibody
产品详细信息
Rabbit anti-Mouse IgG2b Recombinant Secondary Antibody, PE is very specific to Mouse IgG2b with no reactivity towards Mouse IgG1, Mouse IgG2a or Mouse IgG3. It has minimum cross reactivity to Human IgG and Guinea Pig IgG. It showed no cross-reactivity to various species immunoglobulins tested.
This recombinant antibody has been engineered with point mutations that eliminate binding to Fc receptors, enabling reduction in background and superior signal to noise.
R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields. In practical applications, the sensitivity of R-PE conjugates is usually 5 to 10 times greater than those of the corresponding fluorescein conjugates. R-PE is a large molecule used for fluorescence-based detection, primarily in flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity, but not photostability.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
