应用 | 建议稀释比 | 已发表文章 |
---|---|---|
免疫印迹 (WB) |
Assay-dependent | - |
酶联免疫吸附实验 (ELISA) |
Assay-dependent | - |
免疫沉淀 (IP) |
Assay-dependent | - |
对照试剂 (Ctrl) |
Assay-dependent | - |
产品规格 | |
---|---|
种属 |
Rat |
表达系统 |
E. coli |
分子量 |
21.7 kDa |
分类 |
Recombinant |
类型 |
Protein |
纯度 |
>98% by SDS-PAGE |
活性 |
ED50 = 0.01 ng/mL; determined by the dose-dependent proliferation of mouse 7TD1 cells. |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
纯化类型 |
purified |
内含物 |
no preservative |
保存条件 |
-20° C, Avoid Freeze/Thaw Cycles |
Rat IL-6 exerts activity in the range of 0.01 to 1.0 ng/mL for most in vitro applications.
Reconstitution and Dilution: Reconstitute in distilled water or buffer at pH >7 to a concentration of 100 ng/µL. After reconstitution, dilute cytokine in a neutral buffer containing carrier protein such as 5-10% FBS or 1-4% high purity BSA (fatty acid free fraction V).
Lyophilized product is stable until expiration if stored desiccated below 0°C. Cytokines diluted as indicated above should be divided into aliquots and stored at -70°C. Recombinant proteins should be stored at concentrations >500 ng/mL to reduce loss of activity.
Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: H-IL-6; IL-6; ILN; Interleukin; Interleukin 6 (interferon, beta 2); Interleukin-6; Interleukin6; M-IL-6; R-IL-6
基因别名: Ifnb2; Il-6; Il6; ILg6
UniProt ID: (Rat) P20607
Entrez Gene ID: (Rat) 24498