Invitrogen

Rbm20 Polyclonal Antibody

Name: Rbm20 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-57404

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2 篇参考文献

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说明书

实验方案

技术支持

说明书

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技术支持

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Rbm20 Antibody in Immunocytochemistry (ICC/IF)

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Rbm20 Antibody (PA5-57404) in ICC/IF

Nature communications 2019 - Fig. 8 TTN transcription is required for nucleation of RBM20 into foci. a Schematic of the hESC gene editing strategies used to test the role of transcription at the TTN locus. Prom: promoter (P) deletion; KO: functional knockout by frameshift mutation (indicated by the X). Aspects of TTN biogenesis predicted to be impaired by each modification are indicated by increased transparency (loss of transcr... View More

Rbm20 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

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Rbm20 Antibody — Fig. 9 Knockout of RBM20 dysregulates alternative splicing in hESC-derived cardiomyocytes. a Predicted effects of the knockout of RBM20 on TTN biogenesis (loss of RBM20-dependent alternative exon exclusion). Refer to the legend of Fig. 8a for the abbreviations. b Representative immunofluorescence results in CM derived from wild-type or RBM20 knockout hESCs generated with two independent CRISPR/Cas9 single guide RNAs (sgRNAs). Nuclei are counterstained with DAPI, and insets show magnified views; scale bars: 10 um. c Western blot validation of RBM20 knockout (uncropped images shown in Supplementary Fig. 11 ). g: sgRNA. d RT-qPCR in CM derived from the indicated hESC lines. Expression is relative to the housekeeping gene RPLP0 and mean +- s.e.m. is shown. n = 5 (WT and RBM20 KO g1) or 2 ( RBM20 KO g2) independent differentiations. e Expected major TTN isoforms in CM. Exons predicted to be excluded by RBM20 are in blue, and arrows indicate the location of RT-qPCR primers. f As in panel ( d ), but expression of the indicated TTN isoform is relative to the total amount of TTN . g Expected CACNA1C isoforms due to RBM20-dependent alternative exon selection. RBM20 is predicted to favor inclusion of exon 9 over exon 9*. h As in panel ( d ), but expression of the CACNA1C exon 9* isoform is relative to the total amount of CACNA1C . i Expected CAMK2D isoforms due to RBM20-dependent alternative exon selection. RBM20 is predicted to favor inclusion of exon 14 over exons 15-16. j Semiquantia {KO}

产品信息

PA5-57404

应用

建议稀释比

已发表文章

免疫印迹 (WB)

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免疫细胞化学 (ICC/IF)

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产品规格

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human Rbm20

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.1 mg/mL

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2646372

产品详细信息

Immunogen sequence: RRAKEDQALL SVRPLQAHEL NDFHGVAPLH LPHICSICDK KVFDLKDWEL HVKGKLHAQK CLVFS

Highest antigen sequence identity to the following orthologs: Mouse - 88%, Rat - 88%.

靶标信息

This gene encodes a protein that likely binds RNA. Mutations in this gene have been associated with familial dilated cardiomyopathy.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Rbm20 Polyclonal Antibody

Rbm20 Antibody (PA5-57404) in ICC/IF

产品信息

PA5-57404

免疫印迹 (WB)

免疫细胞化学 (ICC/IF)

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

保存条件

运输条件

RRID

产品详细信息

靶标信息

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