Invitrogen

RhoGDI Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他9个RhoGDI抗体

说明书

Protocols

技术支持

说明书

Protocols

技术支持

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RhoGDI Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of RhoGDI Polyclonal Antibody was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with RhoGDI Rabbit Polyclonal Antibody (Product # 51-1000Z) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

RhoGDI Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF-7 (Lane 1), HeLa (Lane 2), HL-60 (Lane 3), K-562 (Lane 4), U-937 (Lane 5), THP-1 (Lane 6) and U-87 MG (Lane 7). The blot was probed with Anti-RhoGDI Rabbit Polyclonal Antibody (Product # 51-1000Z, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 28 kDa band corresponding to RhoGDI was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

RhoGDI Antibody in Immunoprecipitation (IP) — Immunoprecipitation (IP) of Rho-GDIa from HeLa cell lysates using (lane 1) Rb anti-Rho-GDIa (Product # 51-1000Z) and (lane 2) an irrelevant antibody, followed by Western blotting with Rb anti-Rho-GDIa (Product # 51-1000Z) (both lanes).

RhoGDI Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with RhoGDI siRNA and loss of signal was observed in Western Blot using Anti-RhoGDI Polyclonal Antibody (Product # 51-1000Z). {KD}

产品信息

51-1000Z

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1-3 µg/mL	-
免疫细胞化学 (ICC/IF)	2 µg/mL	-
酶联免疫吸附实验 (ELISA)	Assay-dependent	-
免疫沉淀 (IP)	Assay-dependent	-

产品规格
种属反应	Bovine, Human, Mouse
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	A 25 amino acid synthetic peptide derived from the N-terminus of the human Rho-GDI-alpha protein. This sequence is identical to the bovine sequence.
偶联物	Unconjugated
形式	Liquid
浓度	0.25 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.4
内含物	0.1% sodium azide
保存条件	-20°C
RRID	AB_2533895

靶标信息

Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: epididymis secretory sperm binding protein Li 47e; GDI-1; GDP-dissociation inhibitor, aplysia RAS-related 1; MGC117248; Rho GDI 1; Rho GDI alpha; Rho GDP dissociation inhibitor (GDI) alpha; Rho GDP-dissociation inhibitor 1; Rho-GDI alpha; unnamed protein product

基因别名: 5330430M07Rik; ARHGDIA; C87222; Gdi-1; Gdi1; GDIA1; HEL-S-47e; NPHS8; Rho-GDI; RhoDGI; RHOGDI; RHOGDI-1

UniProt ID: (Human) P52565, (Bovine) P19803, (Mouse) Q99PT1

Entrez Gene ID: (Human) 396, (Bovine) 338054, (Mouse) 192662

分子生物学功能: G-protein modulator