Invitrogen

SAA2 Polyclonal Antibody

查看其他3个SAA2抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (3)

SAA2 Antibody in Immunocytochemistry (ICC/IF)

SAA2 Antibody in Immunocytochemistry (ICC/IF)

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SAA2 Antibody (PA5-115862) in ICC/IF

Immunocytochemistry analysis of SAA2 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115862) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).

SAA2 Antibody in Immunocytochemistry (ICC/IF)

SAA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SAA2 Antibody in Western Blot (WB)

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SAA2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of SAA2 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115862) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).

SAA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of SAA2 in human liver cancer. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115862) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

SAA2 Antibody in Western Blot (WB) — Western blot analysis of SAA2 in human liver (left lane: treated with blocking peptide). Samples were incubated with polyclonal antibody (Product # PA5-115862).

Immunogen image

SAA2 Polyclonal Antibody

Immunogen Image SAA2 Polyclonal Antibody

产品信息

PA5-115862

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human SAA2(Accession P0DJI9), corresponding to amino acid residues T5-H55.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total Serum Amyloid A2.

靶标信息

Major acute phase reactant. [UniProt]

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: SAA2; Serum amyloid A-2 protein; Serum amyloid A1 protein

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基因别名: SAA2

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UniProt ID: (Human) P0DJI9

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Entrez Gene ID: (Human) 6289

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chemoattractant activity apolipoprotein

acute-phase response positive chemotaxis cell chemotaxis