Search
Invitrogen
SAP97 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 2
SAP97 Antibody (PA1-044) in WB
Western blot analysis was performed on membrane extracts (30 µglysate) of HeLa (Lane 1), and MCF-7 (Lane 2). The blots were probed with Rabbit Anti-SAP97 Polyclonal Antibody (Product # PA1-044, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 110 kDa band corresponding to SAP97 was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-044
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues E(16) E Y R S K L S Q T E D R Q L R S S(33) of human SAP97.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-044 detects synapse-associated protein 97 (SAP97) from rat and human tissues. This antibody does not detect other synapse-associated protein family members.
PA1-044 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~140 kDa protein representing SAP97 from rat brain homogenate.
The PA1-044 immunogen is a synthetic peptide corresponding to residues E(16) E Y R S K L S Q T E D R Q L R S S(33) of human SAP97. This sequence is completely conserved in rat and mouse. PA1-044 immunizing peptide (Cat. # PEP-022) is available for use in neutralization and control experiments.
Target Information
This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene, but the full-length nature of some of the variants is not known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Disks large homolog 1; DKFZp761P0818; DKFZp781B0426; Drosophila discs-large tumor suppressor homologue (synapse associated protein); hDlg; SAP-97; Synapse-associated protein 97
Gene Aliases: DLG1; Dlgh1; SAP97
UniProt ID: (Rat) Q62696
Entrez Gene ID: (Rat) 25252
guanylate kinase activity
protein binding
protein C-terminus binding
potassium channel regulator activity
protein kinase binding
phosphatase binding
PDZ domain binding
mitogen-activated protein kinase kinase binding
protein complex scaffold
ionotropic glutamate receptor binding
ion channel binding
L27 domain binding
scaffold/adaptor protein
ureteric bud development
branching involved in ureteric bud morphogenesis
immunological synapse formation
endothelial cell proliferation
lens development in camera-type eye
T cell cytokine production
actin filament organization
cell adhesion
chemical synaptic transmission
nervous system development
protein localization
positive regulation of cell proliferation
single organismal cell-cell adhesion
peristalsis
positive regulation of actin filament polymerization
cortical actin cytoskeleton organization
membrane raft organization
regulation of myelination
activation of protein kinase activity
regulation of protein localization
T cell activation
negative regulation of T cell proliferation
regulation of membrane potential
amyloid precursor protein metabolic process
receptor clustering
positive regulation of potassium ion transport
establishment or maintenance of epithelial cell apical/basal polarity
negative regulation of mitotic cell cycle
nucleotide phosphorylation
reproductive structure development
embryonic skeletal system morphogenesis
tissue morphogenesis
smooth muscle tissue development
negative regulation of epithelial cell proliferation
negative regulation of protein kinase B signaling
hard palate development
bicellular tight junction assembly
protein localization to plasma membrane
positive regulation of establishment of protein localization to plasma membrane
receptor localization to synapse
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support