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Invitrogen
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
1:500-1:10,000 | - |
免疫组化（石蜡） (IHC (P)) |
1:100-1:1,000 | - |
免疫细胞化学 (ICC/IF) |
1:500 | - |
染色质免疫沉淀 实验 (ChIP) |
2.5 µg/10^6 cells | - |
| 产品规格 | |
|---|---|
种属反应 |
Human, Mouse, Rat |
宿主/亚型 |
Rabbit / IgG |
分类 |
Polyclonal |
类型 |
Antibody |
抗原 |
Recombinant fragment corresponding to a region within amino acids 39 and 379 of Human SATB1 |
偶联物 |
Unconjugated |
形式 |
Liquid |
浓度 |
0.78 mg/mL |
纯化类型 |
Antigen affinity chromatography |
保存液 |
0.1M tris glycine, pH 7, with 20% glycerol |
内含物 |
0.01% thimerosal |
保存条件 |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2547636 |
Recommended positive controls: Jurkat, MDA-MB-231, THP-1, mouse thymus, mouse brain, rat brain.
Predicted reactivity: Mouse (100%), Rat (100%), Chicken (98%), Rhesus Monkey (100%), Bovine (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
SATB1 (DNA-binding protein SATB1) is a crucial silencing factor that contributes to the initiation of X inactiviation mediated by Xist RNA that occurs during embryogenesis and in lymphoma. SATB1 binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence, and at nuclear matrix- or scaffold-associated regions. It is thought to recognize the sugar-phosphate structure of double-stranded DNA. SATB1 is also a transcriptonal repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner by binding to matrix attachment regions of DNA and inducing a local chromatin-loop remodeling. SATB1 modulates the genes that are essential in the maturatio of the immune T-cell CD8SP from thymocytes. Alu-like motifs and SATB1-binding sites provide an unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL1 and IL2RA in T-cells by binding to the same domain as HDAC1.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: DNA-binding protein SATB1; special AT-rich sequence binding protein 1; special AT-rich sequence binding protein 1 (binds to nuclear matrix/scaffold-associating DNA); Special AT-rich sequence-binding protein 1
基因别名: 2610306G12Rik; AW413156; SATB1
UniProt ID: (Human) Q01826, (Mouse) Q60611
Entrez Gene ID: (Human) 6304, (Mouse) 20230, (Rat) 316164
分子生物学功能:
helix-turn-helix transcription factor
DNA-binding transcription factor
Lambda repressor-like transcription factor
gene-specific transcriptional regulator
