Invitrogen

SATB2 Polyclonal Antibody

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SATB2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of SATB2 was performed using K-562 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SATB2 Polyclonal Antibody (Product # PA5-19630) at 3 µg/mL concentration in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

SATB2 Antibody in Western Blot (WB) — Western blot was performed using Anti-SATB2 Polyclonal Antibody, (Product # PA5-19630) and a 82 kDa band corresponding to SATB2 was observed in the cell lines tested along with uncharacterized bands at 50 and 60 kDa. Modified whole cell extracts (1%SDS) (40 µg lysate) of U-2 OS (Lane 1), THP-1 (Lane 2) and K-562 (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.6 ug/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

产品信息

PA5-19630

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1 µg/mL	-
免疫细胞化学 (ICC/IF)	3 µg/mL	-

产品规格
种属反应	Human, Mouse, Rat
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Mouse SATB2.
偶联物	Unconjugated
形式	Liquid
浓度	1.0 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.4
内含物	0.02% sodium azide
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_10982525

产品详细信息

PA5-19630 targets SATB2 in IHC (F) and WB applications and shows reactivity with mouse and Rat samples.

The PA5-19630 immunogen is synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Mouse SATB2.

PA5-19630 detects SATB2 which has a predicted molecular weight of approximately 85 kDa.

靶标信息

DNA-binding protein SATB2 binds to DNA at the nuclear matrix- or scaffold associated regions. It is thought to recognize the sugar-phosphate structure of double-stranded DNA. SATB2 is a transcription factor controlling nuclear gene expression by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. It also acts as a docking site for several chromatin remodeling enzymes by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. SATB2 is required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes - probably by modulating BCL11B expression. It is a repressor of Ctip2 and a regulatory determinant of corticocortical connections in the developing cerebral cortex. Mutations in the gene are associate with cleft palate isolated (CPI).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: DNA-binding protein SATB2; FLJ21474; FLJ32076; KIAA1034-like DNA binding protein; MGC119474; MGC119477; SATB family member 2; special AT-rich sequence binding protein 2; Special AT-rich sequence-binding protein 2; two cut domains-containing homeodomain protein

基因别名: GLSS; KIAA1034; mKIAA1034; RGD1562369; SATB2

UniProt ID: (Human) Q9UPW6, (Mouse) Q8VI24

Entrez Gene ID: (Human) 23314, (Mouse) 212712, (Rat) 501145

分子生物学功能: helix-turn-helix transcription factor DNA-binding transcription factor Lambda repressor-like transcription factor gene-specific transcriptional regulator