Invitrogen

SERPINB2 Polyclonal Antibody

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SERPINB2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of PAI2 in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min, using a PAI2 polyclonal antibody (Product # PA5-27857). Green: Primary antibody at a dilution of 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

SERPINB2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of PAI2 in paraffin-embedded NCI-N87 xenograft using a PAI2 polyclonal antibody (Product # PA5-27857) at a dilution of 1:500.

SERPINB2 Antibody in Western Blot (WB) — Western Blot analysis of SERPINB2 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a SERPINB2 Polyclonal Antibody (Product # PA5-27857) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

SERPINB2 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of PAI2 in U87-MG whole cell lysates using 5 µg of PAI2 polyclonal antibody (Product # PA5-27857).

产品信息

PA5-27857

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:20,000	-
免疫组化（石蜡） (IHC (P))	1:100-1:1,000	-
免疫细胞化学 (ICC/IF)	1:100-1:1,000	-
免疫沉淀 (IP)	1:100-1:500	-

产品规格
种属反应	Human, Mouse
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Recombinant fragment corresponding to a region within amino acids 78 and 408 of Human PAI2
偶联物	Unconjugated
形式	Liquid
浓度	0.39 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7, with 20% glycerol
内含物	0.025% Proclin 300
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2545333

产品详细信息

Recommended positive controls: A431, JC, U87-MG.

Predicted reactivity: Mouse (80%), Rabbit (86%), Sheep (81%), Bovine (82%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

PAI2 inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Monocyte Arg-serpin; PAI-2; Placental plasminogen activator inhibitor; Plasminogen activator inhibitor 2; Plasminogen activator inhibitor 2, macrophage; plasminogen activator inhibitor, type II; plasminogen activator inhibitor, type II (arginine-serpin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 2; serine (or cysteine) proteinase inhibitor, clade B, member 2; Serpin B2; serpin peptidase inhibitor, clade B (ovalbumin), member 2; Urokinase inhibitor

基因别名: HsT1201; ovalbumin; PAI; PAI-2; PAI2; PLANH2; SERPINB2

UniProt ID: (Human) P05120, (Mouse) P12388

Entrez Gene ID: (Human) 5055, (Mouse) 18788

分子生物学功能: protease inhibitor