Invitrogen

SET Polyclonal Antibody

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SET Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of SET in Human Adrenal Gland using a SET monoclonal antibody (Product #PA5-17962) at 3.75 µg/mL. The Human Adrenal Gland tissue section was paraffin embeded and detected using steamed antigen retrieval with citrate buffer pH 6, AP-staining.

SET Antibody in Western Blot (WB) — Western blot was performed using Anti-SET Polyclonal Antibody (Product # PA5-17962) and a 38 kDa corresponding to SET was observed across cell lines tested except MCF 10A. The expression of SET is low in MCF 10A when compared to MCF-7 and MDA-MB-231 as it is overexpressed in breast cancers. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), MCF-7 (Lane 2), MDA-MB-231 (Lane 3), MOLT-4 (Lane 4), Jurkat (Lane 5) and MCF 10A (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.3 µg/mL) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

产品信息

PA5-17962

应用	建议稀释比	已发表文章
免疫印迹 (WB)	0.3-1 µg/mL	-

产品规格
种属反应	Human
宿主/亚型	Goat / IgG
分类	Polyclonal
类型	Antibody
抗原	Synthetic peptide sequence (SAPAAKVSKKELNS) corresponding to the N-terminus amino acids of SET
偶联物	Unconjugated
形式	Liquid
浓度	0.5 mg/mL
纯化类型	Antigen affinity chromatography
保存液	TBS, pH 7.3, with 0.5% BSA
内含物	0.02% sodium azide
保存条件	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_10984416

产品详细信息

This antibody is predicted to react with bovine based on sequence homology.

靶标信息

SET is a multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: HLA-DR-associated protein II; I IGAAD; I-2PP2A; IGAAD; Inhibitor of granzyme A-activated DNase; inhibitor-2 of protein phosphatase-2A; OTTHUMP00000022286; PHAPII; Phosphatase 2A inhibitor I2PP2A; protein phosphatase type 2A inhibitor; Protein SET; RP11-216B9.3; SET nuclear oncogene; SET translocation (myeloid leukemia-associated); TAF-1a; TAF-1b; TAF-I; Template-activating factor I; Template-Activating Factor-I, chromatin remodelling factor

基因别名: 2PP2A; I2PP2A; IGAAD; IPP2A2; PHAPII; SET; TAF-I; TAF-IBETA

UniProt ID: (Human) Q01105

Entrez Gene ID: (Human) 6418

分子生物学功能: chromatin/chromatin-binding, or -regulatory protein

功能

DNA replication nucleosome assembly nucleosome disassembly nucleocytoplasmic transport viral process regulation of protein phosphatase type 2A activity negative regulation of histone acetylation negative regulation of catalytic activity regulation of mRNA stability negative regulation of neuron apoptotic process negative regulation of transcription, DNA-templated

参与通路

DNA binding protein phosphatase inhibitor activity protein binding protein phosphatase type 2A regulator activity histone binding

SET Polyclonal Antibody

产品信息

PA5-17962

免疫印迹 (WB)

种属反应

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

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生物信息学

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