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Invitrogen
SF1 Polyclonal Antibody
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SF1 Antibody (PA5-79984) in ICC/IF
Immunocytochemistry analysis of splicing factor 1 using anti-splicing factor 1 antibody (Product # PA5-79984). splicing factor 1 was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5 μg/mL rabbit anti-splicing factor 1 antibody (Product # PA5-79984) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 m... View More
产品信息
PA5-79984
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence at the N-terminus of human splicing factor 1 (11-30aa DFPSKKRKRSRWNQDTMEQK).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.05mg sodium azide, 0.05mg thimerosal
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human K562 whole cell, human A549 whole cell, human THP-1 whole cell, rat PC-12 whole cell, mouse RAW2647 whole cell. IHC: Human Lung Cancer tissue, rat small intestine tissue IHC-F: Rat Spleen tissue. ICC/IF: U87 cell, Hela cell. Flow: 293T cell.
靶标信息
Splicing factor 1 is a nuclear pre-mRNA splicing factor. The protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: BBP; M ZFM1; Mammalian branch point-binding protein; SF; Splicing factor 1; Transcription factor ZFM1; Zinc finger gene in MEN1 locus; Zinc finger protein 162
基因别名: BBP; D11S636; MBBP; MZFM; SF1; WBP4; ZCCHC25; ZFM1; Zfp162; ZNF162
UniProt ID: (Human) Q15637
Entrez Gene ID: (Human) 7536, (Mouse) 22668, (Rat) 117855
spliceosomal complex assembly
mRNA 3'-splice site recognition
mRNA splicing, via spliceosome
transcription, DNA-templated
regulation of transcription, DNA-templated
male sex determination
Leydig cell differentiation
negative regulation of smooth muscle cell proliferation
regulation of steroid biosynthetic process
negative regulation of nucleic acid-templated transcription
mRNA processing
RNA splicing
