Invitrogen

SFMBT1 Polyclonal Antibody

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4 篇参考文献

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Product Image Gallery

SFMBT1 Antibody in Western Blot (WB) — Western Blot using SFMBT1 Polyclonal Antibody (Product # PA5-22393). Sample (30 µg of whole cell lysate). Lane A: IMR32. 7.5% SDS PAGE. SFMBT1 Polyclonal Antibody (Product # PA5-22393) diluted at 1:3,000.

SFMBT1 Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP) — Fig. 6 HMG20A and SFMBT1 interact in vitro. A After 48 h transfection, the co-localization (yellow) of HMG20A (green) and SFMBT1 (red) were analyzed using fluorescent microscopy. Cell nuclei were stained by DAPI (blue). B Interaction of HMG20A and SFMBT1 in cells verified by co-immunoprecipitation. C , D Western blot analysis ( C ) and qRT-PCR ( D ) detect HMG20A protein and mRNA of downstream gene and protein after SFMBT1 knockdown. E , F Western blot and qRT-PCR analysis SFMBT1, HMG20A, EZH2 and FOXO1 protein ( E ) and mRNA levels ( F ) after SFMBT1 or HMG20A knockdown in SW620 and HCT-116 cells. * P < 0.05, ** P < 0.01, & P < 0.05, && P < 0.01.

SFMBT1 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Distribution of SFMBT1 in colorectal cancer (CRC) tumor tissues and cells. A Western blot analysis of SFMBT1 protein levels in normal and tumor tissues. ( n = 6) ( B ) Expression of SFMBT1 in normal and CRC tumor tissues were examined by immunohistochemistry. C Expression of SFMBT1 in CRC tissue microarrays. D , E TCGA data shows the expression of SFMBT1 in tumor tissues and normal tissues or in different cancer stages. F , G qRT-PCR ( F ) and western blot analysis ( G ) detect SFMBT1 mRNA and protein in human colorectal epithelial cells CCD18C0 and CRC cell lines HCT-116, SW620 and SW480.

产品信息

PA5-22393

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化 (IHC)

-

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免疫细胞化学 (ICC/IF)

-

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免疫沉淀 (IP)

-

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 13 and 225 of SFMBT1 (Uniprot ID#Q9UHJ3)

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偶联物

Unconjugated

形式

Liquid

浓度

0.6 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_11152461

产品详细信息

Recommended positive controls: NT2D1, IMR32, Jurkat, HeLa.

Predicted reactivity: Mouse (88%), Rat (87%), Bovine (97%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene shares high similarity with the Drosophila Scm (sex comb on midleg) gene. It encodes a protein which contains four malignant brain tumor repeat (mbt) domains and may be involved in antigen recognition.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

SFMBT1 facilitates colon cancer cell metastasis and drug resistance combined with HMG20A.

Cell death discovery

Pan R,Yu D,Hu J,Yang X,Wang C,Zhang L,Xue P,Sun J,Zhang X,Cai W

Published figure using SFMBT1 polyclonal antibody (Product # PA5-22393) in Immunocytochemistry

Mon May 16 00:00:00 EDT 2022

SFMBT1 facilitates colon cancer cell metastasis and drug resistance combined with HMG20A.

Cell death discovery

Pan R,Yu D,Hu J,Yang X,Wang C,Zhang L,Xue P,Sun J,Zhang X,Cai W

Published figure using SFMBT1 polyclonal antibody (Product # PA5-22393) in Immunocytochemistry

Mon May 16 00:00:00 EDT 2022

SFMBT1 facilitates colon cancer cell metastasis and drug resistance combined with HMG20A.

Cell death discovery

Pan R,Yu D,Hu J,Yang X,Wang C,Zhang L,Xue P,Sun J,Zhang X,Cai W

Published figure using SFMBT1 polyclonal antibody (Product # PA5-22393) in Immunocytochemistry

Mon May 16 00:00:00 EDT 2022

SFMBT1 facilitates colon cancer cell metastasis and drug resistance combined with HMG20A.

Cell death discovery

Pan R,Yu D,Hu J,Yang X,Wang C,Zhang L,Xue P,Sun J,Zhang X,Cai W

Published figure using SFMBT1 polyclonal antibody (Product # PA5-22393) in Immunocytochemistry

Mon May 16 00:00:00 EDT 2022

SFMBT1 facilitates colon cancer cell metastasis and drug resistance combined with HMG20A.

Cell death discovery

Pan R,Yu D,Hu J,Yang X,Wang C,Zhang L,Xue P,Sun J,Zhang X,Cai W

Published figure using SFMBT1 polyclonal antibody (Product # PA5-22393) in Immunocytochemistry

Mon May 16 00:00:00 EDT 2022

生物信息学

蛋白别名: hSFMBT; Renal ubiquitous protein 1; Scm-like with four MBT domains protein 1; Scm-related gene containing four mbt domains; Scm-related gene product containing four mbt domains

基因别名: hSFMBT; RU1; SFMBT; SFMBT1

UniProt ID: (Human) Q9UHJ3

Entrez Gene ID: (Human) 51460

分子生物学功能: chromatin/chromatin-binding, or -regulatory protein

transcription, DNA-templated spermatogenesis covalent chromatin modification cell differentiation negative regulation of transcription, DNA-templated negative regulation of muscle organ development

参与通路

transcription corepressor activity protein binding histone binding