Invitrogen

SFRP1 Polyclonal Antibody

查看其他14个SFRP1抗体

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SFRP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of SFRP1 in paraffin-embedded human colon cancer tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with SFRP1 polyclonal antibody (Product # PA5-95634) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

SFRP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of SFRP1 in paraffin-embedded human lung cancer tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with SFRP1 polyclonal antibody (Product # PA5-95634) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

SFRP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of SFRP1 in paraffin-embedded mouse intestine tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with SFRP1 polyclonal antibody (Product # PA5-95634) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

SFRP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of SFRP1 in paraffin-embedded rat intestine tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with SFRP1 polyclonal antibody (Product # PA5-95634) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

SFRP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of SFRP1 in paraffin-embedded mouse kidney tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with SFRP1 polyclonal antibody (Product # PA5-95634) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

SFRP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of SFRP1 in paraffin-embedded rat kidney tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with SFRP1 polyclonal antibody (Product # PA5-95634) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

SFRP1 Antibody in Western Blot (WB) — Western blot analysis of SFRP1 in Lane 1: human A549 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse HEPA1-6 whole cell lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with SFRP1 polyclonal antibody (Product # PA5-95634) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).

产品信息

PA5-95634

应用	建议稀释比	已发表文章
免疫印迹 (WB)	0.1-0.5 µg/mL	-
免疫组化（石蜡） (IHC (P))	0.5-1 µg/mL	-
酶联免疫吸附实验 (ELISA)	0.1-0.5 µg/mL	-

产品规格
种属反应	Human, Mouse, Rat
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	E.coli-derived human SFRP1 recombinant protein (A204-K314). 查看免疫原
偶联物	Unconjugated
形式	Lyophilized
浓度	500 µg/mL
纯化类型	Affinity chromatography
保存液	PBS with 4mg trehalose
内含物	0.05mg sodium azide
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2807436

产品详细信息

Reconstitution information: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

靶标信息

Secreted frizzled-related protein 1 (SFRP1) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: frizzled-related protein; FRP-1; SARP-2; Secreted apoptosis-related protein 2; Secreted frizzled-related protein 1; secreted frizzled-related sequence protein 1; sFRP-1

基因别名: 2210415K03Rik; AW011917; AW107218; AW742929; FRP; FRP-1; FRP1; FrzA; SARP2; sFRP-1; SFRP1

UniProt ID: (Human) Q8N474, (Mouse) Q8C4U3

Entrez Gene ID: (Human) 6422, (Mouse) 20377, (Rat) 84402

分子生物学功能: transmembrane signal receptor

osteoblast differentiation ureteric bud development positive regulation of cell-matrix adhesion hematopoietic progenitor cell differentiation proteolysis G-protein coupled receptor signaling pathway positive regulation of cell proliferation negative regulation of cell proliferation male gonad development female gonad development cellular response to starvation dorsal/ventral axis specification regulation of cell cycle process negative regulation of gene expression negative regulation of epithelial to mesenchymal transition neural crest cell fate commitment response to organic cyclic compound menstrual cycle phase positive regulation of Wnt signaling pathway negative regulation of Wnt signaling pathway negative regulation of ossification positive regulation of cell growth negative regulation of cell growth negative regulation of cell migration negative regulation of BMP signaling pathway negative regulation of osteoblast proliferation somatic stem cell population maintenance response to drug positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of JUN kinase activity positive regulation of GTPase activity cellular response to fibroblast growth factor stimulus stromal-epithelial cell signaling involved in prostate gland development negative regulation of B cell differentiation positive regulation of fat cell differentiation negative regulation of osteoblast differentiation negative regulation of osteoclast differentiation regulation of angiogenesis positive regulation of smoothened signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated negative regulation of insulin secretion negative regulation of bone remodeling negative regulation of fibroblast proliferation digestive tract morphogenesis positive regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation negative regulation of peptidyl-tyrosine phosphorylation positive regulation of stress fiber assembly positive regulation of focal adhesion assembly canonical Wnt signaling pathway hematopoietic stem cell differentiation bone trabecula formation prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis regulation of branching involved in prostate gland morphogenesis negative regulation of androgen receptor signaling pathway cellular response to vitamin D cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to growth factor stimulus cellular response to prostaglandin E stimulus cellular response to estrogen stimulus cellular response to estradiol stimulus cellular response to hypoxia cellular response to X-ray cellular response to heparin dopaminergic neuron differentiation cellular response to transforming growth factor beta stimulus cellular response to BMP stimulus negative regulation of canonical Wnt signaling pathway planar cell polarity pathway involved in neural tube closure Wnt signaling pathway involved in somitogenesis convergent extension involved in somitogenesis positive regulation of canonical Wnt signaling pathway positive regulation of extrinsic apoptotic signaling pathway via death domain receptors regulation of dopaminergic neuron differentiation midbrain morphogenesis negative regulation of planar cell polarity pathway involved in axis elongation positive regulation of non-canonical Wnt signaling pathway negative regulation of Wnt signaling pathway involved in dorsal/ventral axis specification negative regulation of canonical Wnt signaling pathway involved in controlling type B pancreatic cell proliferation negative regulation of fibroblast apoptotic process positive regulation of fibroblast apoptotic process somitogenesis neural tube closure multicellular organism development anterior/posterior pattern specification Wnt signaling pathway neural tube development actin cytoskeleton organization hemopoiesis cell differentiation regulation of ossification substrate adhesion-dependent cell spreading development of primary male sexual characteristics Wnt signaling pathway, planar cell polarity pathway regulation of establishment of planar polarity positive regulation of extrinsic apoptotic signaling pathway non-canonical Wnt signaling pathway

参与通路

cysteine-type endopeptidase activity G-protein coupled receptor activity frizzled binding protein binding drug binding heparin binding Wnt-protein binding identical protein binding Wnt-activated receptor activity molecular_function