Invitrogen

SH2D5 Polyclonal Antibody

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问题与答案

问题与答案

问题与答案

抗体检测数据 (3)

SH2D5 Antibody in Immunocytochemistry (ICC/IF)

SH2D5 Antibody in Immunocytochemistry (ICC/IF)

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SH2D5 Antibody (PA5-101883) in ICC/IF

Immunocytochemistry analysis of SH2D5 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with SH2D5 polyclonal antibody (Product # PA5-101883) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

SH2D5 Antibody in Immunocytochemistry (ICC/IF)

SH2D5 Antibody in Western Blot (WB)

SH2D5 Antibody in Western Blot (WB)

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SH2D5 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of SH2D5 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with SH2D5 polyclonal antibody (Product # PA5-101883) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

SH2D5 Antibody in Western Blot (WB) — Western blot analysis of SH2D5 extracts from COLO205 cells (heat-shock treatment). Samples were incubated with SH2D5 polyclonal antibody (Product # PA5-101883) using a dilution of 1:1,000. Observed bands: 37 kDa.

SH2D5 Antibody in Western Blot (WB) — Western blot analysis of SH2D5 in 293 cell lysate. Samples were incubated with SH2D5 polyclonal antibody (Product # PA5-101883).

Immunogen image

SH2D5 Polyclonal Antibody

Immunogen Image SH2D5 Polyclonal Antibody

产品信息

PA5-101883

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

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免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human SH2D5(Accession Q6ZV89), corresponding to amino acid residues A367-A417.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total SH2D5.

靶标信息

May be involved in synaptic plasticity regulation through the control of Rac-GTP levels.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: SH2 domain containing 5; SH2 domain-containing protein 5; vascular SH2 domain-containing protein

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基因别名: BC036961; RGD1305952; SH2D5

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UniProt ID: (Human) Q6ZV89, (Mouse) Q8JZW5

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Entrez Gene ID: (Human) 400745, (Rat) 366489, (Mouse) 230863

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protein binding scaffold/adaptor protein

biological_process