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Invitrogen
SHP-1 Recombinant Rabbit Monoclonal Antibody (3H20L13)
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
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SHP-1 Antibody (703568) in ICC/IF
For immunofluorescence analysis, Jurkat cells were fixed and permeabilized for detection of endogenous SHP-1 using Anti-SHP-1 Recombinant Rabbit Monoclonal Antibody (Product # 703568, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Panel a-... View More
产品信息
703568
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
3H20L13
抗原
Protein corresponding to human SHP-1 [aa243-aa541]
偶联物
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2809241
产品详细信息
This antibody is predicted to react with Monkey, Horse, Cat.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
SHP-1 (PTPN6) is a non-receptor protein tyrosine phosphatase that is expressed primarily in hematopoietic cells. The enzyme is composed of two SH2 domains, a tyrosine phosphatase catalytic domain, and a carboxy-terminal regulatory domain. SHP-1 removes phosphates from target proteins to downregulate several tyrosine kinase-regulated pathways. In hematopoietic cells, the amino-terminal SH2 domain of SHP-1 binds to tyrosine phosphorylated erythropoietin receptors (EpoR) to negatively regulate hematopoietic growth. Overexpression of SHP-1 in epithelial cells results in dephosphorylation of the Ros receptor tyrosine kinase and subsequent downregulation of Ros-dependent cell proliferation and transformation. Following ligand binding in myeloid cells, SHP-1 associates with the IL-3R beta chain and downregulates IL-3-induced tyrosine phosphorylation and cell proliferation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: EC 3.1.3.48; hematopoietic cell phosphatase; Hematopoietic cell protein-tyrosine phosphatase; Protein-tyrosine phosphatase 1C; Protein-tyrosine phosphatase SHP-1; PTN6; PTP-1C; SH-PTP1; Tyrosine-protein phosphatase non-receptor type 6
基因别名: HCP; HCPH; HPTP1C; PTP-1C; PTP1C; PTPN6; SH-PTP1; SHP-1; SHP-1L; SHP1
UniProt ID: (Human) P29350
Entrez Gene ID: (Human) 5777
hematopoietic progenitor cell differentiation
negative regulation of humoral immune response mediated by circulating immunoglobulin
protein dephosphorylation
apoptotic process
G-protein coupled receptor signaling pathway
cell proliferation
positive regulation of cell proliferation
negative regulation of cell proliferation
positive regulation of phosphatidylinositol 3-kinase signaling
peptidyl-tyrosine phosphorylation
cell differentiation
platelet activation
platelet formation
T cell costimulation
abortive mitotic cell cycle
positive regulation of cell adhesion mediated by integrin
peptidyl-tyrosine dephosphorylation
intracellular signal transduction
megakaryocyte development
negative regulation of T cell proliferation
natural killer cell mediated cytotoxicity
negative regulation of MAP kinase activity
regulation of B cell differentiation
negative regulation of peptidyl-tyrosine phosphorylation
B cell receptor signaling pathway
negative regulation of B cell receptor signaling pathway
negative regulation of T cell receptor signaling pathway
leukocyte migration
regulation of release of sequestered calcium ion into cytosol
regulation of type I interferon-mediated signaling pathway
regulation of ERK1 and ERK2 cascade
platelet aggregation
regulation of G1/S transition of mitotic cell cycle
