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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • SIRT1 Antibodies

          Invitrogen

          SIRT1 Polyclonal Antibody

          高级验证
          2个已发表图片
          6 篇参考文献
          查看其他40个SIRT1抗体

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          说明书
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          说明书
          实验方案
          问题与答案
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          Cite SIRT1 Polyclonal Antibody

          • 抗体检测数据 (3)
          • 已发表图片 (2)
          • 高级验证 (1)
          SIRT1 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          SIRT1 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          图: 1 / 6

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          SIRT1 Antibody (PA5-17074) in WB

          Western blot was performed using Anti-SIRT1 Polyclonal Antibody (Product # PA5-17074) and a 110kDa band corresponding to SIRT1 was observed across all cell lines and tissues tested. Nuclear enriched extracts (50 µg lysate) of C2C12 (Lane 1), Neuro-2a (Lane 2), Mouse Ovary (Lane 3) and Mouse Testis (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

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          SIRT1 Antibody in Western Blot (WB)
          SIRT1 Antibody in Western Blot (WB)
          SIRT1 Antibody in Western Blot (WB)
          SIRT1 Antibody in Western Blot (WB)
          SIRT1 Antibody in Western Blot (WB)
          SIRT1 Antibody

          产品信息

          PA5-17074

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:1,000
          查看5篇已发表文章 5篇已发表文章

          免疫沉淀 (IP)

          1:50
          查看1篇已发表文章 1篇已发表文章
          产品规格

          种属反应

          Mouse

          已发表种属

          Human, Mouse

          宿主/亚型

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          抗原

          Synthetic peptide corresponding to the carboxy terminus of mouse SirT1
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          316 µg/mL

          纯化类型

          Antigen affinity chromatography

          保存液

          0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

          内含物

          no preservative

          保存条件

          -20°C

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10979977

          产品详细信息

          It is not recommended to aliquot this antibody.

          This antibody is not cross-reactive with other sirtuin proteins.

          靶标信息

          NAD-dependent protein deacetylase sirtuin-1 (SIRT1) links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, repsonse to DNA damage, metabolism, apoptosis, and autophagy. SIRT1 can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histone and DNA, leading to transcriptional repression. It is essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5'-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT).

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: mSIR2a; NAD-dependent deacetylase sirtuin-1; NAD-dependent protein deacetylase sirtuin-1; NAD-dependent protein deacylase sirtuin-1; OTTHUMP00000198111; OTTHUMP00000198112; Regulatory protein SIR2 homolog 1; RP11-57G10.3; Sir2; sir2-like 1; SIR2-like protein 1; SIR2alpha; SIRT 1; SIRT-1; sirtuin 1 ((silent mating type information regulation 2, homolog) 1

          查看更多 收起

          基因别名: AA673258; Sir2; Sir2a; Sir2alpha; SIR2L1; Sirt1

          查看更多 收起

          UniProt ID: (Mouse) Q923E4

          查看更多 收起

          Entrez Gene ID: (Mouse) 93759

          查看更多 收起

          功能
          core promoter sequence-specific DNA binding p53 binding transcription corepressor activity histone deacetylase activity protein binding protein C-terminus binding transcription factor binding protein-N-terminal asparagine amidohydrolase activity UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity hydrolase activity NAD-dependent histone deacetylase activity iprodione amidohydrolase activity (3,5-dichlorophenylurea)acetate amidohydrolase activity deacetylase activity enzyme binding protein domain specific binding protein deacetylase activity 4'-(2-hydroxyisopropyl)phenylurea amidohydrolase activity didemethylisoproturon amidohydrolase activity N-isopropylacetanilide amidohydrolase activity N-cyclohexylformamide amidohydrolase activity isonicotinic acid hydrazide hydrolase activity cis-aconitamide amidase activity gamma-N-formylaminovinylacetate hydrolase activity NAD-dependent protein deacetylase activity histone binding identical protein binding HLH domain binding protein kinase B binding bHLH transcription factor binding N2-acetyl-L-lysine deacetylase activity O-succinylbenzoate synthase activity indoleacetamide hydrolase activity N-acetylcitrulline deacetylase activity metal ion binding NAD-dependent histone deacetylase activity (H3-K9 specific) N-acetylgalactosamine-6-phosphate deacetylase activity mitogen-activated protein kinase binding diacetylchitobiose deacetylase activity chitooligosaccharide deacetylase activity NAD+ binding keratin filament binding chromatin/chromatin-binding, or -regulatory protein histone modifying enzyme
          参与通路
          single strand break repair negative regulation of transcription from RNA polymerase II promoter chromatin silencing at rDNA pyrimidine dimer repair by nucleotide-excision repair DNA synthesis involved in DNA repair angiogenesis ovulation from ovarian follicle cellular glucose homeostasis positive regulation of protein phosphorylation positive regulation of adaptive immune response chromatin organization establishment of chromatin silencing maintenance of chromatin silencing transcription, DNA-templated regulation of transcription, DNA-templated rRNA processing protein deacetylation triglyceride mobilization apoptotic process cellular response to DNA damage stimulus response to oxidative stress multicellular organism development spermatogenesis regulation of mitotic cell cycle muscle organ development positive regulation of cell proliferation intrinsic apoptotic signaling pathway in response to DNA damage cellular response to starvation positive regulation of heart rate negative regulation of gene expression negative regulation of cardiac muscle cell apoptotic process positive regulation of cholesterol efflux regulation of glucose metabolic process positive regulation of neuron projection development positive regulation of skeletal muscle cell proliferation positive regulation of macroautophagy protein ubiquitination histone deacetylation peptidyl-lysine acetylation cell differentiation negative regulation of cell growth negative regulation of transforming growth factor beta receptor signaling pathway negative regulation of prostaglandin biosynthetic process protein destabilization positive regulation of chromatin silencing negative regulation of TOR signaling regulation of endodeoxyribonuclease activity negative regulation of NF-kappaB transcription factor activity negative regulation of tumor necrosis factor production response to insulin circadian regulation of gene expression regulation of protein import into nucleus, translocation regulation of smooth muscle cell apoptotic process peptidyl-lysine deacetylation cellular triglyceride homeostasis regulation of peroxisome proliferator activated receptor signaling pathway positive regulation of insulin secretion involved in cellular response to glucose stimulus regulation of cell proliferation negative regulation of phosphorylation response to hydrogen peroxide behavioral response to starvation cholesterol homeostasis intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of I-kappaB kinase/NF-kappaB signaling proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of sequence-specific DNA binding transcription factor activity negative regulation of DNA damage response, signal transduction by p53 class mediator positive regulation of MHC class II biosynthetic process response to ethanol negative regulation of fat cell differentiation positive regulation of gluconeogenesis positive regulation of DNA repair negative regulation of transcription, DNA-templated positive regulation of vasodilation positive regulation of transcription from RNA polymerase II promoter positive regulation of insulin receptor signaling pathway rhythmic process white fat cell differentiation negative regulation of helicase activity positive regulation of histone H3-K9 methylation negative regulation of protein kinase B signaling fatty acid homeostasis negative regulation of growth hormone secretion negative regulation of cell death negative regulation of androgen receptor signaling pathway histone H3-K9 modification cellular response to hydrogen peroxide regulation of bile acid biosynthetic process UV-damage excision repair histone H3 deacetylation cellular response to tumor necrosis factor negative regulation of histone H3-K14 acetylation cellular response to hypoxia cellular response to ionizing radiation stress-induced premature senescence negative regulation of neuron death negative regulation of protein acetylation negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway negative regulation of reactive oxygen species biosynthetic process histone H3-K9 deacetylation positive regulation of macrophage apoptotic process negative regulation of fibroblast apoptotic process negative regulation of cAMP-dependent protein kinase activity positive regulation of cAMP-dependent protein kinase activity regulation of energy homeostasis positive regulation of thyroid-stimulating hormone secretion negative regulation of histone H4-K16 acetylation negative regulation of cellular response to testosterone stimulus negative regulation of peptidyl-lysine acetylation negative regulation of cellular senescence positive regulation of cellular senescence
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