Invitrogen

SLC7A11 Polyclonal Antibody

Product Image Gallery

SLC7A11 Antibody in Western Blot (WB) — Western blot was performed using Anti-SLC7A11 Polyclonal Antibody (Product # PA1-16775) and a 55 kDa band corresponding to SLC7A11 was observed across the cell lines and tissue tested. An uncharacterized band of ~ 36 kDa was also observed in some of the cell lines. Whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane1), MCF7 (Lane 2), Hep G2 (Lane 3), HeLa (Lane 4), HCT 116 (Lane 5), K-562 (Lane 6), U-87MG (Lane 7) and tissue extract (30 µg lysate) of Mouse Stomach (Lane 8) were electrophoresed using Novex® NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution), using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

产品信息

PA1-16775

应用	建议稀释比	已发表文章
免疫印迹 (WB)	0.5-2 µg/mL	-
免疫细胞化学 (ICC/IF)	1:100-1:1,000	-

产品规格
种属反应	Human, Mouse
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	A synthetic peptide made to a region within the N-terminus of the murine XCT protein sequence (between residues 1-50).
偶联物	Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS
内含物	0.02% sodium azide
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_568777

产品详细信息

This antibody is predicted to react with mouse and canine based on 100% sequence homology.

This antibody has 94% sequence homology with rat.

Suggested positive control: human stomach protein, stomach tissue lysate.

靶标信息

SLC7A11 (also known as XCT) is a member of a hetromeric, sodium-independent, anionic amino acid transport system. It is highly specific for cysteine and glutamate. SLC7A11 is a mediator of Kaposi sarcoma-associated herpes virus fusion and entry into cells. Increased expression of this protein in primary gliomas is associated with neuronal cell death.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: Amino acid transport system xc-; Calcium channel blocker resistance protein CCBR1; cysteine/glutamate transporter; Cystine/glutamate transporter; sodium independent anionic amino acid transport system; solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11; Solute carrier family 7 member 11; solute carrier family 7, (cationic amino acid transporter, y+ system) member 11; xCT

基因别名: 9930009M05Rik; AI451155; CCBR1; SLC7A11; sut; xCT

UniProt ID: (Human) Q9UPY5, (Mouse) Q9WTR6

Entrez Gene ID: (Human) 23657, (Mouse) 26570

分子生物学功能: transporter