Invitrogen

SLK Polyclonal Antibody

Product Image Gallery

SLK Antibody in Western Blot (WB) — Western blot analysis of SLK in Lane 1: human HeLa whole cell lysates, Lane 2: human PANC-1 whole cell lysates, Lane 3: human 22RV1 whole cell lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with SLK polyclonal antibody (Product # PA5-95518) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).

SLK Antibody in Flow Cytometry (Flow) — Flow cytometry of SLK in U251 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with SLK polyclonal antibody (Product # PA5-95518) at a dilution of 1 µg (per 1x10^6 cells), followed by 488 conjugated goat anti-rabbit IgG (30 min at 20°C) using a 5-10 µg (per 1x10^6 cells) dilution.

产品细节

Applications

Tested Dilution

Publications

免疫印迹 (WB)

0.1-0.5 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

产品规格

种属反应

Rat, Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human SLK recombinant protein (Position: M1-E232).

偶联物

Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2807320

产品详细信息

Reconstitution information: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

靶标信息

SLK mediates apoptosis and actin stress fiber dissolution.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: CTCL tumor antigen se20-9; Etk4; hSLK; Long Ste20-like Kinase; serine/threonine kinase 2; Serine/threonine-protein kinase 2; SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase, SNF1 sucrose nonfermenting like kinase; SNF1 sucrose nonfermenting like kinase; STE20-like kinase; STE20-like serine/threonine-protein kinase; STE20-related kinase; STE20-related kinase SMAK; Ste20-related serine/threonine kinase; STE20-related serine/threonine-protein kinase

基因别名: 9A2; AV021402; AW411554; bA16H23.1; Etk4; KIAA0204; LOSK; mKIAA0204; mSLK; se20-9; Sk2; SLK; SMAK; STK2

UniProt ID: (Human) Q9H2G2, (Mouse) O54988, (Rat) O08815

Entrez Gene ID: (Human) 9748, (Mouse) 20874, (Rat) 54308

功能