Invitrogen

SLK Polyclonal Antibody

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SLK Antibody in Western Blot (WB) — Western blot analysis of SLK in Lane 1: human HeLa whole cell lysates, Lane 2: human PANC-1 whole cell lysates, Lane 3: human 22RV1 whole cell lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with SLK polyclonal antibody (Product # PA5-95518) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).

SLK Antibody in Flow Cytometry (Flow) — Flow cytometry of SLK in U251 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with SLK polyclonal antibody (Product # PA5-95518) at a dilution of 1 µg (per 1x10^6 cells), followed by 488 conjugated goat anti-rabbit IgG (30 min at 20°C) using a 5-10 µg (per 1x10^6 cells) dilution.

产品信息

PA5-95518

应用	建议稀释比	已发表文章
免疫印迹 (WB)	0.1-0.5 µg/mL	-
流式细胞分析 (Flow)	1-3 µg/1x10^6 cells	-
酶联免疫吸附实验 (ELISA)	0.1-0.5 µg/mL	-

产品规格
种属反应	Human, Mouse, Rat
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	E.coli-derived human SLK recombinant protein (Position: M1-E232).
偶联物	Unconjugated
形式	Lyophilized
浓度	500 µg/mL
纯化类型	Affinity chromatography
保存液	PBS with 4mg trehalose
内含物	0.05mg sodium azide
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2807320

产品详细信息

Reconstitution information: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

靶标信息

SLK mediates apoptosis and actin stress fiber dissolution.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CTCL tumor antigen se20-9; Etk4; hSLK; Long Ste20-like Kinase; serine/threonine kinase 2; Serine/threonine-protein kinase 2; SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase, SNF1 sucrose nonfermenting like kinase; SNF1 sucrose nonfermenting like kinase; STE20-like kinase; STE20-like serine/threonine-protein kinase; STE20-related kinase; STE20-related kinase SMAK; Ste20-related serine/threonine kinase; STE20-related serine/threonine-protein kinase

基因别名: 9A2; AV021402; AW411554; bA16H23.1; Etk4; KIAA0204; LOSK; mKIAA0204; mSLK; se20-9; Sk2; SLK; SMAK; STK2

UniProt ID: (Human) Q9H2G2, (Mouse) O54988, (Rat) O08815

Entrez Gene ID: (Human) 9748, (Mouse) 20874, (Rat) 54308

功能

apoptotic process signal transduction by protein phosphorylation regulation of cell migration cytoplasmic microtubule organization regulation of apoptotic process protein autophosphorylation regulation of focal adhesion assembly cell-cell adhesion protein phosphorylation regulation of mitotic cell cycle phosphorylation stress-activated protein kinase signaling cascade activation of protein kinase activity positive regulation of histone phosphorylation positive regulation of apoptotic process intracellular signal transduction cellular response to tumor necrosis factor

参与通路

protein serine/threonine kinase activity receptor signaling protein serine/threonine kinase activity protein binding ATP binding identical protein binding protein homodimerization activity cadherin binding involved in cell-cell adhesion nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

SLK Polyclonal Antibody

产品信息

PA5-95518

免疫印迹 (WB)

流式细胞分析 (Flow)

酶联免疫吸附实验 (ELISA)

种属反应

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

篇参考文献 (0)

生物信息学

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