Invitrogen

SMAD4 Recombinant Superclonal Antibody (10HCLC)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

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产品信息

710714

应用
建议稀释比
已发表文章

免疫印迹 (WB)

0.5-2 µg/mL
-

免疫细胞化学 (ICC/IF)

2 µg/mL
-
产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Superclonal

类型

Antibody

克隆号

10HCLC

抗原

full-length protein corresponding to human SMAD4 (aa 1-552)
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2532757

产品详细信息

This antibody is predicted to react with Monkey, Cat, Horse, Pig, Bovine, Rabbit and Goat.

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

靶标信息

Smad4 is encoded by the Smad4 gene that is located on chromosome 18 in humans. Smad4 is also known as Smad family member 4, Sma- and Mad-related protein 4 or Mothers against decapentaplegic (MAD) homolog 4. Smad4 contains N-terminal MH1 (MAD homology 1) and C-terminal MH2 (MAD homology 2) globular domains that are involved in DNA binding and protein interactions respectively. Binding of the TGF-beta superfamily of ligands that includes Transforming Growth Factor -beta (TGF-beta) and bone morphogenetic protein (BMP) to its cognate receptor allows phosphorylation of Smad 1, 2, 3, 5 and 8 (R-Smad, Receptor Smad). This signals for heterotrimerization with Smad4 (co-Smad, co-mediator Smad) and translocation of the complex to the nucleus. Inhibitory or antagonistic Smad (I-Smad) that includes Smad 6 and 7, interact with activated R-Smads and attenuate the signaling pathway. Smad4 acts as a tumor suppressor protein by transcriptionally regulating its target genes such as Cyclin D1 (downregulation) and collagen (upregulation) that inhibit cell proliferation. Dephosphorylation regulates nuclear export and nucleocytoplasmic dynamics of Smads.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Deleted in Pancreatic Carcinoma 4 (DPC4); deleted in pancreatic carcinoma locus 4; Deletion target in pancreatic carcinoma 4; hSMAD4; MAD homolog 4; MAD homolog 4 (MADH4); Mothers against decapentaplegic homolog 4; Mothers against decapentaplegic homolog 4 (MADH4); mothers against decapentaplegic, Drosophila, homolog of, 4; SMAD; SMAD 4; SMAD family member 4; SMAD, mothers against DPP homolog 4

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基因别名: DPC4; JIP; MADH4; MYHRS; SMAD4

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UniProt ID: (Human) Q13485

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Entrez Gene ID: (Human) 4089

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功能
RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding transcription factor activity, protein binding transcription factor activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding collagen binding transforming growth factor beta receptor, common-partner cytoplasmic mediator activity identical protein binding protein homodimerization activity sequence-specific DNA binding transcription regulatory region DNA binding metal ion binding protein heterodimerization activity I-SMAD binding R-SMAD binding DNA-binding transcription factor gene-specific transcriptional regulator
参与通路
negative regulation of transcription from RNA polymerase II promoter ovarian follicle development branching involved in ureteric bud morphogenesis response to hypoxia in utero embryonic development gastrulation with mouth forming second atrioventricular valve formation epithelial to mesenchymal transition involved in endocardial cushion formation left ventricular cardiac muscle tissue morphogenesis positive regulation of cell proliferation involved in heart valve morphogenesis brainstem development transcription from RNA polymerase II promoter cellular iron ion homeostasis transforming growth factor beta receptor signaling pathway SMAD protein complex assembly spermatogenesis single fertilization axon guidance endoderm development mesoderm development cell proliferation negative regulation of cell proliferation negative regulation of cardiac muscle hypertrophy positive regulation of epithelial to mesenchymal transition positive regulation of pathway-restricted SMAD protein phosphorylation neural crest cell differentiation regulation of transforming growth factor beta receptor signaling pathway negative regulation of cell growth BMP signaling pathway positive regulation of transforming growth factor beta receptor signaling pathway positive regulation of BMP signaling pathway somite rostral/caudal axis specification regulation of transforming growth factor beta2 production positive regulation of luteinizing hormone secretion somatic stem cell population maintenance intracellular signal transduction atrioventricular canal development endothelial cell activation embryonic digit morphogenesis negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of follicle-stimulating hormone secretion developmental growth neuron fate commitment sebaceous gland development formation of anatomical boundary regulation of binding positive regulation of histone H3-K4 methylation regulation of hair follicle development palate development uterus development positive regulation of SMAD protein import into nucleus SMAD protein signal transduction ventricular septum morphogenesis negative regulation of cell death endocardial cell differentiation female gonad morphogenesis interleukin-6-mediated signaling pathway negative regulation of ERK1 and ERK2 cascade response to transforming growth factor beta cellular response to BMP stimulus metanephric mesenchyme morphogenesis nephrogenic mesenchyme morphogenesis seminiferous tubule development positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus positive regulation of histone H3-K9 acetylation
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