Invitrogen

SMARCB1 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. 查看细节

查看其他12个SMARCB1抗体

说明书

Protocols

技术支持

说明书

Protocols

技术支持

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SMARCB1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of SMARCB1 in U2OS cells. Samples were incubated with SMARCB1 polyclonal antibody (Product # PA5-76820).

SMARCB1 Antibody in Western Blot (WB) — Western blot was performed using Anti-SMARCB1 Polyclonal Antibody (Product # PA5-76820) and a ~44 kDa band was observed across the panel tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of A549 (Lane 1), PC-3 (Lane 2), HeLa (Lane 3), K-562 (Lane 4), HEK-293 (Lane 5), MCF-7 (Lane 6), U-2 OS (Lane 7), tissue extracts (30 µg lysate) of Mouse Spleen (Lane 8) and Rat Spleen (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

SMARCB1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with SMARCB1 siRNA and a decrease in signal intensity was observed in western blot application using Anti-SMARCB1 Polyclonal Antibody (Product # PA5-76820). {KD}

产品细节

Applications

Tested Dilution

Publications

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:50-1:200

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant full length Human SMARCB1.

偶联物

Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 50% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2720547

产品详细信息

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

靶标信息

The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2alpha) and Brg-1 (also designated SNF2beta) are the ATPase subunits of the mammalian SWI-SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI-SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/SNF, including the NURF (nucleosome remodeling factor).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: BAF47; BRG1-associated factor 47; hSNF5; Integrase interactor 1 protein; malignant rhabdoid tumor suppressor; mSNF5; protein phosphatase 1, regulatory subunit 144; SNF5 homolog; sucrose nonfermenting, yeast, homolog-like 1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1; SWI/SNF-related matrix-associated protein; SWI10; TYE4

基因别名: AU020204; BAF47; CSS3; hSNFS; INI1; MRD15; PPP1R144; RDT; RTPS1; Sfh1p; SMARCB1; SNF5; SNF5/INI1; SNF5L1; Snr1; SWNTS1

UniProt ID: (Human) Q76N08, (Mouse) Q9Z0H3

Entrez Gene ID: (Human) 6598, (Mouse) 20587, (Rat) 361825

分子生物学功能: DNA metabolism protein nucleic acid metabolism protein