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Invitrogen
SMYD1 Polyclonal Antibody
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SMYD1 Antibody (PA5-96630) in WB
Western blot analysis of SMYD1 in extracts of various cell lines. Samples were incubated with SMYD1 Polyclonal antibody (Product # PA5-96630) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-96630
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human SMYD1 (NP_9380151)
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.39 mg/mL
规格
39 µg
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: MTIGRMENVE VFTAEGKGRG LKATKEFWAA DIIFAERAYS AVVFDSLVNF VCHTCFKRQE KLHRCGQCKF AHYCDRTCQK DAWLNHKNEC SAIKRYGKVP NENIRLAARI MWRVEREGTG LTEGCLVSVD DLQNHVEHFG EEEQKDLRVD VDTFLQYWPP QSQQFSMQYI SHIFGVINCN GFTLSDQRGL QAVGVGIFPN LGLVNHDCWP NCTVIFNNGN HEAVKSMFHT QMRIELRALG KISEGEELTV SYIDFLNVSE ERKRQLKKQY YFDCTCEHCQ KKLKDDLFLG VKDNPKPSQE VVKEMIQFSK DTLEKIDKAR SEGLYHEVVK LCRECLEKQE PVFADTNIYM; Positive Samples: HepG2, Raji, MCF7, Mouse skeletal muscle, Rat heart, Rat skeletal muscle; Cellular Location: Cytoplasm, Nucleus
靶标信息
Acts as a transcriptional repressor. Essential for cardiomyocyte differentiation and cardiac morphogenesis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD8b-opposite; CD8beta opposite strand; Histone-lysine N-methyltransferase Smyd1; m-BOP; SET and MYND domain-containing protein 1; Zinc finger protein BOP
基因别名: 4632404M21Rik; Bop; C78565; Smyd1; Zmynd18
UniProt ID: (Mouse) P97443
Entrez Gene ID: (Mouse) 12180, (Rat) 297333
chromatin remodeling
transcription, DNA-templated
regulation of transcription, DNA-templated
heart development
positive regulation of myotube differentiation
methylation
skeletal muscle cell differentiation
positive regulation of myoblast differentiation
negative regulation of transcription, DNA-templated
biological_process
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