Invitrogen

SNIP1 Polyclonal Antibody

查看其他6个SNIP1抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (2)

SNIP1 Antibody in Western Blot (WB)

SNIP1 Antibody in Western Blot (WB)

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SNIP1 Antibody (PA5-112273) in WB

Western Blot analysis of SNIP1 using SNIP1 Polyclonal Antibody (Product # PA5-112273) at a dilution of 1:500. Lane A: Jurkat whole cell lysate, (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 46 kDa. Observed band size: 52 kDa.

SNIP1 Antibody in Western Blot (WB)

SNIP1 Antibody in Immunoprecipitation (IP)

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SNIP1 Antibody in Western Blot (WB) — Western Blot analysis of SNIP1 using SNIP1 Polyclonal Antibody (Product # PA5-112273) at a dilution of 1:500. Lane A: Jurkat whole cell lysate, (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 46 kDa. Observed band size: 52 kDa.

SNIP1 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of SNIP1 was performed on (Lane A) 0.5 mg Jurkat whole cell lysate using 4 µL of SNIP1 Polyclonal Antibody (Product # PA5-112273) at a dilution of 1:100, and 60 µg of Immunomagnetic beads Protein A/G. A Clean-Blot IP Detection Reagent (HRP) was used as a secondary antibody at a dilution of 1:1,000. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 46 kDa. Observed band size: 50 kDa.

产品信息

PA5-112273

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫沉淀 (IP)

1-5 µL/mg of lysate

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived Human SNIP1 fragment

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography, Protein A

保存液

PBS, pH 7

内含物

0.03% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

Smad-binding peptide aptamers can be developed to selectively inhibit TGF-beta-induced gene expression.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: dJ423B22.2; FHA domain-containing protein SNIP1; RP3-423B22.3; Smad nuclear-interacting protein 1

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基因别名: PMRED; SNIP1

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UniProt ID: (Human) Q8TAD8

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Entrez Gene ID: (Human) 79753

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protein binding poly(A) RNA binding RNA splicing factor

regulation of transcription, DNA-templated I-kappaB kinase/NF-kappaB signaling production of miRNAs involved in gene silencing by miRNA positive regulation of protein targeting to mitochondrion