Invitrogen

SOCS6 Polyclonal Antibody

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SOCS6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of SOCS6 in paraffin-embedded Human liver cancer tissue using SOCS6 Polyclonal Antibody (Product # PA5-50263) at a 1:50 dilution. (Original magnification: x200).

SOCS6 Antibody in Western Blot (WB) — EV2 Figure MiR-342-3p directly targets SOCS6 to regulate anti-Mtb immunity A Relative expressions of miR-342-2p target genes were analyzed by qRT-PCR. Data are shown as the mean +- SEM of n = 3 biological replicates. B Cell death mechanisms of RAW264.7 cells transfected with siRNA, followed by Mtb infection for 36 h. Representative data (from n = 3 biological replicates) are shown as the mean +- SEM of technical replicates. C, D MiR-342-3p mimic or inhibitor was transfected to RAW264.7 macrophages. After 48 h, cells were collected for qRT-PCR (C, data are shown as the mean +- SEM of n = 3 biological replicates) and Western blotting (D, representative blots from n = 3 biological replicates are shown) to detect the relative levels of SOCS6. E C3H BMDMs were transfected with miR-342-3p mimic or SOCS6-overexpressing lentivirus, followed by Mtb infection. Cell death mechanisms were analyzed, respectively. Representative data (from n = 3 biological replicates) are shown as the mean +- SEM of technical replicates. F B6 BMDMs were transfected with miR-342-3p inhibitor or Socs6 siRNA, followed by Mtb infection. Cell death mechanisms were analyzed, respectively. Representative data (from n = 3 biological replicates) are shown as the mean +- SEM of technical replicates. G, H SOCS6-overexpressing vector (G) or Socs6 siRNA (H) was mixed with polyethylenimine to form a complex, which was used to infect mice by tail vein injection ( N /P ratio=8). Lungs were collected for transfection effic

产品信息

PA5-50263

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:200-1:1,000	查看2篇已发表文章 2篇已发表文章
免疫组化（石蜡） (IHC (P))	1:50-1:200	-

产品规格
种属反应	Human, Mouse
已发表种属	Not Applicable
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Fusion protein corresponding to a region derived from internal residues of human suppressor of cytokine signaling 6
偶联物	Unconjugated
形式	Liquid
浓度	0.7 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.4, with 40% glycerol
内含物	0.05% sodium azide
保存条件	-20°C
RRID	AB_2635716

产品详细信息

The antibody detects endogenous levels of total SOCS-6 protein.

靶标信息

The eight members of the recently identified Suppressor of Cytokines Signaling (SOCS) family are SOCS1, SOCS2, SOCS3, SOCS4, SOCS5, SOCS6, SOCS7, and CIS. Structurally the SOCS proteins are composed of an N- terminal region of variable length and amino acid composition, a central SH2 domain, and a C-terminal motif called the SOCS box. The SOCS proteins appear to form part of a classical negative feedback loop that regulates cytokine signal transduction. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. SOCS1 and SOCS6 interact with the insulin receptor (IR) when expressed in human hepatoma cells (HepG2) or in rat hepatoma cells overexpressing human IR. SOCS1 and SOCS6 inhibit insulin-dependent activation of ERK1/2 and protein kinase B in vivo and IR- directed phosphorylation of IRS1 in vitro. These results suggest that SOCS proteins may be inhibitors of IR signalling and could mediate cytokine-induced insulin resistance and contribute to the pathogenesis of type II diabetes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

MiR-342 controls Mycobacterium tuberculosis susceptibility by modulating inflammation and cell death.

EMBO reports

Fu B,Lin X,Tan S,Zhang R,Xue W,Zhang H,Zhang S,Zhao Q,Wang Y,Feldman K,Shi L,Zhang S,Nian W,Chaitanya Pavani K,Li Z,Wang X,Wu H

Published figure using SOCS6 polyclonal antibody (Product # PA5-50263) in Western Blot

Mon Sep 06 00:00:00 EDT 2021

A short deletion in the DNA-binding domain of STAT3 suppresses growth and progression of colon cancer cells.

Aging

Xiong YJ,Liu DY,Shen RR,Xiong Y

Published figure using SOCS6 polyclonal antibody (Product # PA5-50263) in Western Blot

Mon Feb 01 00:00:00 EST 2021

生物信息学

蛋白别名: CIS-4; cytokine inducible SH2-containing protein 4; cytokine inducible SH2-containing protein CIS4; Cytokine-inducible SH2 protein 4; SH2 domain containing SOCS box protein SOCS6; SOCS-4; SOCS-6; STAT induced STAT inhibitor-4; Suppressor of cytokine signaling 4; Suppressor of cytokine signaling 6

基因别名: 1500012M23Rik; 5830401B18Rik; AI447482; CIS-4; CIS4; Cish4; HSPC060; SOCS-4; SOCS-6; SOCS4; SOCS6; SSI4; STAI4; STATI4

UniProt ID: (Human) O14544, (Mouse) Q9JLY0

Entrez Gene ID: (Human) 9306, (Mouse) 54607

分子生物学功能: kinase modulator defense/immunity protein