Invitrogen

SOD1 Monoclonal Antibody (8B10)

SOD1 Antibody in Immunocytochemistry (ICC/IF)

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SOD1 Antibody (MA1-105) in ICC/IF

Immunofluorescence analysis of SOD1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SOD1 (8B10) Mouse Monoclonal Antibody (Product # MA1-105) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175)... View More

SOD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SOD1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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SOD1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with SOD1 siRNA and decrease in signal intensity was observed in western blot application using Anti-SOD1 Mouse Monoclonal Antibody (Product# MA1105). {KD}

产品信息

MA1-105

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

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免疫组化（石蜡） (IHC (P))

1:20

免疫细胞化学 (ICC/IF)

2 µg/mL

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免疫沉淀 (IP)

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse, Rat

宿主/亚型

Mouse / IgG

分类

Monoclonal

类型

Antibody

克隆号

8B10

抗原

Full length human recombinant protein of human SOD1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 1mg/mL BSA, 30% glycerol

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2536811

产品详细信息

MA1-105 detects SOD1 in human and mouse samples and has been successfully used in Western blot, immunohistochemistry, immunocytochemistry and immunofluorescence applications.

靶标信息

SOD1 (superoxide dismutase) binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

ALS-linked SOD1 mutations impair mitochondrial-derived vesicle formation and accelerate aging.

Redox biology

Guo Y,Guan T,Yu Q,Sanghai N,Shafiq K,Li M,Jiao X,Na D,Zhang G,Kong J

Published figure using SOD1 monoclonal antibody (Product # MA1-105) in Western Blot

Thu Feb 01 00:00:00 UTC 2024

Identification of high-performing antibodies for Superoxide dismutase [Cu-Zn] 1 (SOD1) for use in Western blot, immunoprecipitation, and immunofluorescence.

F1000Research

Ayoubi R,Alshafie W,You Z,Southern K,McPherson PS,Laflamme C

Published figure using SOD1 monoclonal antibody (Product # MA1-105) in Western Blot

Mon Oct 23 00:00:00 UTC 2023

Identification of high-performing antibodies for Superoxide dismutase [Cu-Zn] 1 (SOD1) for use in Western blot, immunoprecipitation, and immunofluorescence.

F1000Research

Ayoubi R,Alshafie W,You Z,Southern K,McPherson PS,Laflamme C

Published figure using SOD1 monoclonal antibody (Product # MA1-105) in Western Blot

Mon Oct 23 00:00:00 UTC 2023

Selective removal of misfolded SOD1 delays disease onset in a mouse model of amyotrophic lateral sclerosis.

Cellular and molecular life sciences : CMLS

Guan T,Zhou T,Zhang X,Guo Y,Yang C,Lin J,Zhang JV,Cheng Y,Marzban H,Wang YT,Kong J

MA1-105 was used in Western Blot to prove that selective removal of misfolded SOD1 is a promising treatment for ALS.

Tue Sep 26 00:00:00 UTC 2023

Antioxidant effect of grape seed extract corrects experimental autoimmune encephalomyelitis behavioral dysfunctions, demyelination, and glial activation.

Frontiers in immunology

Mabrouk M,El Ayed M,Démosthènes A,Aissouni Y,Aouani E,Daulhac-Terrail L,Mokni M,Bégou M

Published figure using SOD1 monoclonal antibody (Product # MA1-105) in Western Blot

Thu Sep 08 00:00:00 UTC 2022

N-Acetylcysteine Enhances the Recovery of Ischemic Limb in Type-2 Diabetic Mice.

Antioxidants (Basel, Switzerland)

Zhu Q,Liu X,Zhu Q,Liu Z,Yang C,Wu H,Zhang L,Xia X,Wang M,Hao H,Cui Y,Zhang G,Hill MA,Flaker GC,Zhou S,Liu Z

MA1-105 was used in Western Blotting to indicate that NAC treatment enhanced the recovery of blood flow and mechanical function in ischemic limbs in T2D mice in association with improved tissue redox/inflammatory status and insulin resistance.

Tue May 31 00:00:00 UTC 2022

Tempol Preserves Endothelial Progenitor Cells in Male Mice with Ambient Fine Particulate Matter Exposure.

Biomedicines

Liu X,Wang A,Chen Z,Cui Y,Hao H,Domeier TL,Sun Q,Liu Z

MA1-105 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to indicate that particulate matter (PM) exposure-induced reduction of endothelial progenitor cell population in male mice may be due to decreased expression of pulmonary superoxide dismutase 1 in male mice.

Sat Jan 29 00:00:00 UTC 2022

Combination of Antioxidant Enzyme Overexpression and N-Acetylcysteine Treatment Enhances the Survival of Bone Marrow Mesenchymal Stromal Cells in Ischemic Limb in Mice With Type 2 Diabetes.

Journal of the American Heart Association

Zhu Q,Hao H,Xu H,Fichman Y,Cui Y,Yang C,Wang M,Mittler R,Hill MA,Cowan PJ,Zhang G,He X,Zhou S,Liu Z

MA1-105 was used in Western Blotting to conclude that simultaneous suppression of reactive oxygen species from transplanted bMSCs and host tissue could additively enhance bMSC survival in diabetic ischemic limb with increased therapeutic efficacy in diabetes.

Tue Oct 05 00:00:00 UTC 2021

Combination of Antioxidant Enzyme Overexpression and N-Acetylcysteine Treatment Enhances the Survival of Bone Marrow Mesenchymal Stromal Cells in Ischemic Limb in Mice With Type 2 Diabetes.

Journal of the American Heart Association

Zhu Q,Hao H,Xu H,Fichman Y,Cui Y,Yang C,Wang M,Mittler R,Hill MA,Cowan PJ,Zhang G,He X,Zhou S,Liu Z

Tue Oct 05 00:00:00 UTC 2021

The copper chaperone CCS facilitates copper binding to MEK1/2 to promote kinase activation.

The Journal of biological chemistry

Grasso M,Bond GJ,Kim YJ,Boyd S,Matson Dzebo M,Valenzuela S,Tsang T,Schibrowsky NA,Alwan KB,Blackburn NJ,Burslem GM,Wittung-Stafshede P,Winkler DD,Marmorstein R,Brady DC

MA1-105 was used in Western Blot to indicate that the Cu chaperone CCS provides fidelity within a complex biological system to achieve appropriate installation of Cu within the MEK1 kinase active site that in turn modulates kinase activity and supports the development of novel MEK1/2 inhibitors that target the Cu structural interface or blunt dedicated Cu delivery mechanisms via CCS.

Wed Dec 01 00:00:00 UTC 2021

Fine tuning of the unfolded protein response by ISRIB improves neuronal survival in a model of amyotrophic lateral sclerosis.

Cell death & disease

Bugallo R,Marlin E,Baltanás A,Toledo E,Ferrero R,Vinueza-Gavilanes R,Larrea L,Arrasate M,Aragón T

Published figure using SOD1 monoclonal antibody (Product # MA1-105) in Immunocytochemistry

Tue May 26 00:00:00 UTC 2020

Fine tuning of the unfolded protein response by ISRIB improves neuronal survival in a model of amyotrophic lateral sclerosis.

Cell death & disease

Bugallo R,Marlin E,Baltanás A,Toledo E,Ferrero R,Vinueza-Gavilanes R,Larrea L,Arrasate M,Aragón T

Published figure using SOD1 monoclonal antibody (Product # MA1-105) in Immunocytochemistry

Tue May 26 00:00:00 UTC 2020

Concomitant overexpression of triple antioxidant enzymes selectively increases circulating endothelial progenitor cells in mice with limb ischaemia.

Journal of cellular and molecular medicine

Liu L,Cui Y,Li X,Que X,Xiao Y,Yang C,Zhang J,Xie X,Cowan PJ,Tian J,Hao H,Liu Z

MA1-105 was used in Western Blotting to determine if ischaemia induced reactive oxygen species differentially regulate individual endothelial progenitor cell populations.

Sat Jun 01 00:00:00 UTC 2019

Mitochondrial superoxide disrupts the metabolic and epigenetic landscape of CD4⁺ and CD8⁺ T-lymphocytes.

Redox biology

Moshfegh CM,Collins CW,Gunda V,Vasanthakumar A,Cao JZ,Singh PK,Godley LA,Case AJ

Published figure using SOD1 monoclonal antibody (Product # MA1-105) in Western Blot

Tue Oct 01 00:00:00 UTC 2019

Protective Effects of Kuding Tea (Ilex kudingcha C. J. Tseng) Polyphenols on UVB-Induced Skin Aging in SKH1 Hairless Mice.

Molecules (Basel, Switzerland)

Yi R,Zhang J,Sun P,Qian Y,Zhao X

MA1-105 was used in Western Blotting to study the protective effects of Kuding tea polyphenols on ultraviolet B-induced skin injury of SKH1 hairless mice.

Wed Mar 13 00:00:00 UTC 2019

生物信息学

蛋白别名: Amyotrophic lateral sclerosis 1 (ALS1); Cu(2+)-Zn2+ superoxide dismutase; Cu/Zn SOD; Cu/Zn superoxide dismutase; epididymis secretory protein Li 44; HGNC:11179; hSod1; indophenoloxidase A; Indophenoloxidase A (IPOA); SOD, soluble; superoxide dismutase; Superoxide dismutase 1; Superoxide Dismutase 1 (SOD1); Superoxide dismutase [Cu-Zn]; superoxide dismutase, cystolic

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基因别名: ALS; ALS1; B430204E11Rik; Cu/Zn-SOD; CuZnSOD; HEL-S-44; homodimer; hSod1; Ipo-1; Ipo1; IPOA; SOD; Sod-1; SOD1; SODC

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UniProt ID: (Human) P00441, (Mouse) P08228

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Entrez Gene ID: (Human) 6647, (Mouse) 20655

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功能