Invitrogen

SOD2 Monoclonal Antibody (1H6)

Name: SOD2 Monoclonal Antibody (1H6)
Brand: Invitrogen
SKU: MA1-106

高级验证

1个已发表图片

7 篇参考文献

查看其他34个SOD2 (MnSOD)抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

SOD2 Antibody in Immunocytochemistry (ICC/IF)

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SOD2 Antibody (MA1-106) in ICC/IF

Immunofluorescence analysis of SOD2 was performed using 70% confluent log phase A549 cells treated with TNF alpha (20 ng/mL for 72h). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SOD2 Polyclonal Antibody (Product # MA1-106) at 2 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluo... View More

SOD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SOD2 Antibody in Immunoprecipitation (IP)

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SOD2 Antibody — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis using SOD2 Polyclonal Antibody (Product # MA1-106) shows increased expression of SOD2 in A549 upon treatment with TNF alpha. {TM}

Immunogen image

Immunogen Image SOD2 Monoclonal Antibody (1H6)

产品信息

MA1-106

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

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免疫组化（石蜡） (IHC (P))

1:100

免疫细胞化学 (ICC/IF)

2 µg/mL

免疫沉淀 (IP)

2 µg

其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG

分类

Monoclonal

类型

Antibody

克隆号

1H6

抗原

Full length recombinant protein of human SOD2

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 1mg/mL BSA, 30% glycerol

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2536812

产品详细信息

MA1-106 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, immunohistochemistry and immunoprecipitation applications with human samples. This antibody is not suitable for mouse liver tissue in IHC applications and is not suitable for PC12 cells in IF applications.

The MA1-106 immunogen is full length recombinant protein of human SOD2.

靶标信息

Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn-SOD/SOD-1), mitochondrial manganese SOD (Mn-SOD/SOD-2) and extracellular Cu, Zn-SOD (EC-SOD/SOD-3). SOD1 is found in all eukaryotic species as a homodimeric 32 kDa enzyme containing one each of Cu and Zn ion per subunit. The manganese containing 80 kDa tetrameric enzyme SOD2, is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain. SOD3 is a heparin-binding multimer of disulfide-linked dimers, primarily expressed in human lungs, vessel walls and airways. SOD4 is a copper chaperone for superoxide dismutase (CCS), which specifically delivers Cu to copper/zinc superoxide dismutase. CCS may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: indophenoloxidase B; IPO B; manganese-containing superoxide dismutase; mangano-superoxide dismutase; Mn superoxide dismutase; superoxide dismutase; Superoxide dismutase [Mn], mitochondrial

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基因别名: IPO-B; IPOB; Mn-SOD; MNSOD; MVCD6; SOD2

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UniProt ID: (Human) P04179

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Entrez Gene ID: (Human) 6648

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功能

superoxide dismutase activity manganese ion binding identical protein binding oxidoreductase metabolite interconversion enzyme

参与通路