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          • Primary Antibodies ›
          • SOX9 Antibodies

          Invitrogen

          SOX9 Recombinant Rabbit Monoclonal Antibody (7H13L8)

          高级验证
          3个已发表图片
          7 篇参考文献
          查看其他49个SOX9抗体

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          Cite SOX9 Recombinant Rabbit Monoclonal Antibody (7H13L8)

          Additional Information:
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          • 抗体检测数据 (6)
          • 已发表图片 (3)
          • 高级验证 (3)
          SOX9 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          SOX9 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          图: 1 / 12

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          SOX9 Antibody (702016) in WB

          Western blot analysis was performed on Modified Whole cell extracts (1% SDS) (30 µg lysate) of C2C12 (Lane 1), NIH/3T3 (Lane 2), HCT 116 (Lane 3) and Caco-2 (Lane 4). The blots were probed with Anti-SOX9 Recombinant Rabbit Monoclonal Antibody (Product # 702016, 2.5 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 70 kDa band corresponding to SOX9 was observed across the cell lines tested. Known quantity of prot... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

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          SOX9 Antibody in Immunocytochemistry (ICC/IF)
          SOX9 Antibody in Immunocytochemistry (ICC/IF)
          SOX9 Antibody in Western Blot (WB)
          SOX9 Antibody in Western Blot (WB)
          SOX9 Antibody in Western Blot (WB)
          SOX9 Antibody in ChIP Assay (ChIP)
          SOX9 Antibody in Immunohistochemistry (IHC)
          SOX9 Antibody in Immunohistochemistry (IHC)
          SOX9 Antibody in Western Blot (WB)
          SOX9 Antibody
          SOX9 Antibody
          SOX9 Antibody

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          SOX9 Recombinant Rabbit Monoclonal Antibody (7H13L8)

          产品信息

          702016

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          2.5 µg/mL
          查看3篇已发表文章 3篇已发表文章

          免疫组化 (IHC)

          -
          查看1篇已发表文章 1篇已发表文章

          免疫组化(冰冻) (IHC (F))

          -
          查看1篇已发表文章 1篇已发表文章

          免疫细胞化学 (ICC/IF)

          1:100
          查看1篇已发表文章 1篇已发表文章

          染色质免疫沉淀 实验 (ChIP)

          2.5 µg/1x10^6 cells
          查看1篇已发表文章 1篇已发表文章
          产品规格

          种属反应

          Human, Mouse

          已发表种属

          Human, Mouse

          宿主/亚型

          Rabbit / IgG

          Expression System

          Expi293

          分类

          Recombinant Monoclonal

          类型

          Antibody

          克隆号

          7H13L8

          抗原

          Peptides corresponding to human SOX9 [1) aa4-aa17 2) aa47-aa62]
          3D表位/免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          0.5 mg/mL

          规格

          100 µg

          纯化类型

          Protein A

          保存液

          PBS, pH 7.2-7.4

          内含物

          0.09% sodium azide

          保存条件

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          运输条件

          Wet ice

          RRID

          AB_2716879

          产品详细信息

          This antibody is predicted to react with Monkey, Pig, Mouse

          Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

          靶标信息

          SOX9 has a role in sex determination and differentiation of Sertoli cells. It is involved in chondrogenesis and regulates the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. Translocation of this gene can cause campomelic dysplasia. SOX9 is involved in the formation of testes from the indifferent fetal gonads. It is a major molecular component of the neuron-glia switch in developing spinal cord. During normal development, SOX9 allows the prostate epithelium to outgrow into the mesenchyme and then provides basal cell support for development and maintenance of the luminal epithelium. These functions of SOX9 are subverted in prostate cancer to support tumor growth and invasion. SOX9 may direct the formation of neural crest precursors and the development of a range of neural crest derivative. It has a transcriptional regulation in melanin production in cells. Northern blot analysis shows its expression on adult testis, adult heart, and fetal brain.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: SOX 9; SOX9; SRY (sex determining region Y)-box9; SRY (sex-determining region Y)-box 9 protein; SRY-box 9; SRY-Box 9 ; SRY-box containing gene 9; SRY-related HMG-box, gene 9; unnamed protein product

          查看更多 收起

          基因别名: 2010306G03Rik; CMD1; CMPD1; ENH13; mKIAA4243; mSox9; SRA1; SRXX2; SRXY10; TESCO

          查看更多 收起

          UniProt ID: (Human) P48436, (Mouse) Q04887

          查看更多 收起

          Entrez Gene ID: (Human) 6662, (Mouse) 20682

          查看更多 收起

          功能
          transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding beta-catenin binding protein kinase A catalytic subunit binding bHLH transcription factor binding sequence-specific DNA binding pre-mRNA intronic binding sequence-specific double-stranded DNA binding
          参与通路
          negative regulation of transcription from RNA polymerase II promoter skeletal system development cartilage condensation ossification branching involved in ureteric bud morphogenesis cell fate specification epithelial to mesenchymal transition tissue homeostasis hair follicle development morphogenesis of an epithelium positive regulation of mesenchymal cell proliferation chondrocyte differentiation negative regulation of immune system process heart valve development heart valve morphogenesis aortic valve morphogenesis endocardial cushion morphogenesis chondrocyte differentiation involved in endochondral bone morphogenesis chondrocyte hypertrophy growth plate cartilage chondrocyte growth nucleosome assembly chromatin remodeling signal transduction epidermal growth factor receptor signaling pathway spermatogenesis central nervous system development heart development positive regulation of cell proliferation male gonad development gene expression regulation of cell cycle process positive regulation of gene expression positive regulation of epithelial cell migration neural crest cell fate specification male germ-line sex determination negative regulation of ossification prostate gland development positive regulation of epithelial cell differentiation mammary gland development notochord development otic vesicle formation negative regulation of chondrocyte differentiation positive regulation of chondrocyte differentiation somatic stem cell population maintenance regulation of cell proliferation regulation of apoptotic process negative regulation of apoptotic process negative regulation of myoblast differentiation negative regulation of osteoblast differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of fatty acid oxidation negative regulation of photoreceptor cell differentiation oligodendrocyte differentiation positive regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation cartilage development Sertoli cell differentiation Sertoli cell development retina development in camera-type eye limb bud formation retinal rod cell differentiation epithelial cell proliferation involved in prostatic bud elongation intestinal epithelial structure maintenance regulation of cell proliferation involved in tissue homeostasis positive regulation of cartilage development morphogenesis of a branching epithelium protein-containing complex assembly negative regulation of biomineral tissue development ERK1 and ERK2 cascade response to fatty acid cellular response to mechanical stimulus cellular response to retinoic acid cellular response to interleukin-1 cellular response to epidermal growth factor stimulus cellular response to heparin cellular response to transforming growth factor beta stimulus otic vesicle development cellular response to BMP stimulus renal vesicle induction metanephric tubule development ureter urothelium development metanephric nephron tubule formation negative regulation of canonical Wnt signaling pathway cochlea morphogenesis positive regulation of kidney development positive regulation of branching involved in ureteric bud morphogenesis positive regulation of chondrocyte proliferation negative regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of beta-catenin-TCF complex assembly positive regulation of male gonad development positive regulation of mesenchymal stem cell differentiation positive regulation of protein phosphorylation chondrocyte development heart valve formation regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter cytoskeleton organization Notch signaling pathway negative regulation of cell proliferation regulation of gene expression negative regulation of gene expression neural crest cell development cell differentiation regulation of cell adhesion extracellular matrix organization male sex determination negative regulation of bone mineralization negative regulation of epithelial cell differentiation endocrine pancreas development lacrimal gland development protein localization to nucleus intrahepatic bile duct development cell fate commitment regulation of cell differentiation positive regulation of protein catabolic process homeostasis of number of cells within a tissue astrocyte fate commitment endochondral bone morphogenesis epithelial tube branching involved in lung morphogenesis lung epithelial cell differentiation prostate gland morphogenesis bronchus cartilage development trachea cartilage development regulation of branching involved in lung morphogenesis lung smooth muscle development Harderian gland development ureter development ureter smooth muscle cell differentiation ureter morphogenesis anterior head development cell-cell adhesion positive regulation of extracellular matrix assembly positive regulation of cell proliferation involved in heart morphogenesis regulation of epithelial cell proliferation involved in lung morphogenesis negative regulation of mesenchymal cell apoptotic process
          It has to be done as per old AB suggested Products section.

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