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Invitrogen
SP6 Polyclonal Antibody
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SP6 Antibody (PA5-80061) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of SP6 in A549 cell using SP6 Polyclonal Antibody (Product # PA5-80061). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL and mouse anti-Beta Tubulin antibody overnight at 4°C. DyLight 488 conjugated donkey anti-rabbit IgG and DyLight 594 conjugated goat anti-mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C.... View More
产品信息
PA5-80061
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence of human SP6 (QPDMSHHYESWFRPTHPGAEDGSWWDLHPGTSWMDLPH).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
4mg trehalose, 0.9mg NaCl, 0.2mg Na2PO4
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human CACO-2 whole cell, human RT4 whole cell, human A431 whole cell, human K562 whole cell. IHC: human lung cancer tissue, human placenta tissue, mouse stomach tissue, rat stomach tissue. ICC/IF: A549 cell.
靶标信息
SP6 belongs to a family of transcription factors that contain 3 classical zinc finger DNA-binding domains consisting of a zinc atom tetrahedrally coordinated by 2 cysteines and 2 histidines (C2H2 motif). These transcription factors bind to GC-rich sequences and related GT and CACCC boxes.SP6 belongs to a family of transcription factors that contain 3 classical zinc finger DNA-binding domains consisting of a zinc atom tetrahedrally coordinated by 2 cysteines and 2 histidines (C2H2 motif). These transcription factors bind to GC-rich sequences and related GT and CACCC boxes (Scohy et al., 2000).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Epiprofin; Krueppel-like factor 14; Kruppel-like factor 14; MGC119662; MGC119663; MGC119664; MGC119665; Sp6 transcription factor; specificity protein 6; trans-acting transcription factor 6; Transcription factor Sp6
基因别名: 1110025J03Rik; AA591031; AI592962; EPFN; EPIPROFIN; KLF14; SP6
UniProt ID: (Human) Q3SY56, (Mouse) Q9ESX2
Entrez Gene ID: (Human) 80320, (Mouse) 83395, (Rat) 363672
