Invitrogen

SP7 Recombinant Rabbit Monoclonal Antibody (PSH07-29)

SP7 Antibody in Immunocytochemistry (ICC/IF)

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SP7 Antibody (MA562409) in ICC/IF

Immunofluorescence analysis of SP7 was performed using 70% confluent log phase Saos-2 and U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SP7 Recombinant Rabbit Monoclonal Antibody (PSH07-29) (Product # MA562409) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2,000 diluti... View More

SP7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SP7 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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SP7 Antibody — Immunohistochemical analysis of SP7/Osterix was performed using formalin-fixed paraffin-embedded mouse bone marrow and mouse heart tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 15 minutes. Following antigen retrieval, the sections were blocked with 3% H2O2 for 1 hour at room temperature followed by 2% normal goat serum in 1X PBS for 45 minutes at room temperature. The sections were then probed with SP7 Recombinant Rabbit monoclonal Antibody (PSH07-29) (Product # MA562409) at 1:500 dilution in 0.1% normal goat serum overnight at 4 degree Celsius in a humidified chamber. Detection was performed using Alexa Fluor™ 594 Tyramide SuperBoost™ Kit, goat anti-rabbit IgG (Product # B40925). Nuclei were stained with DAPI (Product # D1306) and the sections were mounted using ProLong™ Glass Antifade Mountant (Product # P36984). The images were captured on EVOS™ M7000 Imaging System (Product # AMF7000) at 20X magnification. Strong staining was observed in high SP7/Osterix expressing mouse bone marrow tissue section and no staining was observed in low expressing mouse heart tissue section. {RE}

产品信息

MA562409

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

免疫组化（石蜡） (IHC (P))

1:200-1:1,000

免疫组化（冰冻） (IHC (F))

1:200

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

PSH07-29

抗原

Recombinant protein within human Sp7 aa 1-300.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Positive Control: Saos-2 cell lysates, human osteosarcoma tissue, mouse embryo tissue, rat embryo tissue, E14.5 mouse embryo tissue.

Tissue Specificity: Not detected.

Subcellular Location: Nucleus.

Sequence Similarities: 95% Mouse.

Predicted band size: 45 kDa.

靶标信息

SP7 is a C2H2-type zinc finger transcription factor of the SP gene family and a putative master regulator of bone cell differentiation (Gao et al., 2004 [PubMed 15474293]).

仅用于科研。不用于诊断过程。未经明确授权不得转售。