Invitrogen

SPIN1 Polyclonal Antibody

图: 1 / 3

SPIN1 Antibody (PA5-96448) in WB

Western blot analysis of SPIN1 in extracts of various cell lines. Samples were incubated with SPIN1 Polyclonal antibody (Product # PA5-96448) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Product Image Gallery

SPIN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of SPIN1 in paraffin-embedded mouse brain. Samples were incubated with SPIN1 Polyclonal antibody (Product # PA5-96448) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

产品信息

PA5-96448

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 1-262 of human SPIN1.

查看免疫原

偶联物

Unconjugated

形式

Liquid

浓度

0.82 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2808250

产品详细信息

Immunogen sequence: MKTPFGKTPG QRSRADAGHA GVSANMMKKR TSHKKHRSSV GPSKPVSQPR RNIVGCRIQH GWKEGNGPVT QWKGTVLDQV PVNPSLYLIK YDGFDCVYGL ELNKDERVSA LEVLPDRVAT SRISDAHLAD TMIGKAVEHM FETEDGSKDE WRGMVLARAP VMNTWFYITY EKDPVLYMYQ LLDDYKEGDL RIMPDSNDSP PAEREPGEVV DSLVGKQVEY AKEDGSKRTG MVIHQVEAKP SVYFIKFDDD FHIYVYDLVK TS; Positive Samples: MCF7, SW480, 293T, Jurkat, HeLa, HepG2, SH-SY5Y, Mouse brain; Cellular Location: Nucleus, nucleolus

靶标信息

SPIN1 (spindlin 1), also known as SPIN or ovarian cancer-related protein (OCR), is a 262 amino acid nuclear protein suggested to play a role in regulation of the cell-cycle during the transition from gamete to embryo. A member of the SPIN/STSY family, SPIN1 localizes to interphase nucleus and mitotic chromosomes, and is modified by phosphorylation in a cell-cycle-dependent fashion. A meiotic spindle-binding protein, SPIN1 overexpression has been shown to cause defects in mitotic spindles, thereby resulting in chromosome instability and potential tumorigenesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。