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Invitrogen
SPSB1 Polyclonal Antibody
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SPSB1 Antibody (PA5-89504) in WB
Western blot analysis of SPSB1 in extracts of various cell lines. Samples were incubated with SPSB1 Polyclonal antibody (Product # PA5-89504) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
产品信息
PA5-89504
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
C. elegans
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-60 of human SPSB1 (NP_0793822)
偶联物
形式
Liquid
浓度
1.83 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.09% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: MGQKVTGGIK TVDMRDPTYR PLKQELQGLD YCKPTRLDLL LDMPPVSYDV QLLHSWNNND; Positive Samples: U-87MG, A-549, A-375, Mouse spleen, Rat testis; Cellular Location: Cytoplasm
靶标信息
Substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:15601820, PubMed:21199876). Negatively regulates nitric oxide (NO) production and limits cellular toxicity in activated macrophages by mediating the ubiquitination and proteasomal degradation of NOS2 (PubMed:21199876). Acts as a bridge which links NOS2 with the ECS E3 ubiquitin ligase complex components ELOC and CUL5 (PubMed:21199876). [UniProt]
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CBG; novel SPRY domain containing protein; SPRY domain-containing SOCS box protein 1; SPRY domain-containing SOCS box protein SSB-1; SSB-1
基因别名: 1110014L01Rik; 4930422J18Rik; AI596360; AI854583; RGD1309319; SPSB1; SSB-1; SSB1
UniProt ID: (Human) Q96BD6, (Mouse) Q9D5L7
Entrez Gene ID: (Human) 80176, (Rat) 313722, (Mouse) 74646
